Neurite-SELEXによって生成されたAnti-L1CAMアプタマーによるニューライトネットワークのイメージング
Linlin Wang1,2, Tao Bing1,2, Ying Liu1,2
1Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences , Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190 , China.
Journal of the American Chemical Society
|November 29, 2018
まとめ
研究者はニューライトと関連する L1CAM タンパク質を標的とした DNA アプタマー yly12 を開発しました このアプタマーはニューラルネットワークを視覚化し,ニューライトの増殖を抑制し,神経科学の研究のための新しいツールを提供します.
科学分野:
- 神経科学
- バイオテクノロジー
- 分子生物学
背景:
- 神経細胞の成長は神経系の発達に不可欠であり,神経毒性と神経再生を評価する重要な要因です.
- ニューライトのための特定の分子プローブの開発は,これらの評価に不可欠です.
研究 の 目的:
- 修飾されたSELEX戦略を使用してニューライトのためのDNAアプタマープローブを開発する.
- アプタメアの分子標的を特定し,神経科学の研究におけるその有用性を評価する.
主な方法:
- ニューライト特有のSELEX (指数的濃縮によるリガンドの系統的進化) 戦略がDNAアプタマーを生成するために使用されました.
- アプタマー yly12が選択され,その結合標的が特定されました.
- アプタマーの有効性は,生細胞イメージング,脳組織セクションイメージング,神経細胞増殖抑制アッセイでテストされました.
主要な成果:
- ニューライトに強く結合するDNAアプタマー yly12が生成された.
- yly12の分子標的は,神経細胞粘着分子L1 (L1CAM) と特定されました.
- yly12は,生きている細胞と脳組織におけるニューライトネットワークのイメージングに成功し,ニューライトの増殖を抑制する効果を示しました.
結論:
- DNAアプタマー yly12は神経科学の研究の強力な分子ツールであり,特にニューライトを視覚化し,その発達を研究するものです.
- ニューライト-SELEX戦略は,サブセルラー構造をターゲットにアプタマー生成に有効です.
- yly12は神経変性疾患の研究や神経再生のための薬の開発に 応用される可能性があります
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