酵母U2 snRNAの2つの保存ドメインは945の非必須核酸によって分離されています
1Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
Cell
|October 7, 1988
まとめ
イーストU2小核RNA (snRNA) は,異なる機能ドメインを持っています. イーストのU2 snRNAの中央部を削除すると,成長には影響がないが,3'端を変更すると,細胞の生命力に影響する.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- バイオケミストリー バイオケミストリー
背景:
- イーストのU2小核RNA (snRNA) は,哺乳類の同類よりも大幅に大きい.
- 酵母U2 snRNAの機能領域を理解することは,細胞プロセスにおけるその役割を明らかにするために不可欠です.
研究 の 目的:
- 大酵母U2 snRNA分子内の異なるドメインの機能的重要性を調査する.
- 細胞の生存能力と機能に必要な酵母U2 snRNAの重要な領域を決定する.
主な方法:
- 消去分析は,酵母U2 snRNA.のセグメントを体系的に除去するために使用されました.
- 成長率の評価を含むフェノタイプ分析は,削除されたU2 snRNA領域を持つ酵母菌株で行われました.
- U2 snRNA 3'端の分析は,3'端領域の欠損がある細胞で行われました.
主要な成果:
- イーストのU2 snRNA分子は,機能的に3つの異なる領域に分けることができます.
- 高度保存された5'ドメインの削除 (約. 120ヌクレオチド) は致命的であり,その重要な役割を確認しています.
- 驚くべきことに,大きな中央ドメイン (945ヌクレオチド) の削除は,酵母菌の成長率に影響を与えない.
- 3'端領域 (110核酸) の配列の除去は,成長を遅らせ,U2 snRNA 3'端を変化させ,安定性における役割を示唆する.
結論:
- 酵母U2 snRNAは,少なくとも2つの機能的に重要な要素を有しています:不可欠な5'ドメインと粒子の形成に関与する3'ドメインです.
- 酵母U2snRNAの5'ドメインは,スプライシング反応に直接関与しています.
- 酵母U2 snRNAの3'ドメインは,小さな核リボ核タンパク質粒子 (snRNP) の生成と安定性において重要な役割を果たしている可能性が高い.
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Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
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