人間の胎盤期における母胎相互作用のモデルとしてのトロフォブラスト・オーガノイド
Margherita Y Turco1,2,3, Lucy Gardner4,5, Richard G Kay6
1Department of Pathology, University of Cambridge, Cambridge, UK. myt25@cam.ac.uk.
Nature
|November 30, 2018
まとめ
研究者は新しいヒトトロフォブラスト オーガノイドモデルを開発しました この発見により 胎盤の発達と機能の 長期的な研究が可能になり 妊娠障害を理解する上で 極めて重要です
科学分野:
- 発達生物学
- 生殖医学
- 幹細胞生物学
背景:
- 人間の胎盤は胎児の発達に不可欠ですが 機能的なモデルが不足しているため 知識は限られています
- 胎盤機能障害は重度の妊娠合併症を引き起こします
- トロフォブラスト細胞は,様々な機能を持つ胎盤の重要な成分です.
研究 の 目的:
- 長期にわたって遺伝的に安定したヒトトロフォブラスト・オルガノイドモデルを作成する.
- トロフォブラストの分化と機能のインビトロ研究を可能にします.
- トロフォブラストと母親の環境の相互作用を調査する.
主な方法:
- ヒトのブラストシストからトロフォブラスト・オーガノイド培養物の生成
- ヒト白血球抗原 (HLA) タイプ化による胎児の起源の確認
- 差別化マーカー,ホルモン分泌 (hCG,GDF15,PSG) と侵襲性の分析
- メチローム分析で,オーガノイドと第1期胎盤を比較する.
主要な成果:
- オルガノイドはシンチオトロフブラストとエクストラビロストロフブラストに 順調に分化した.
- オルガノイドは状の構造を形成し,重要な胎盤ホルモンを分泌しました.
- エクストラビヨス・トロフォブラスト・オーガノイドは3D培養で強力な侵入を示した.
- オーガノイドメチロームは 胎盤に似ていた
結論:
- 新しい機能的なヒトトロフォブラスト・オルガノイドモデルが確立されました.
- このモデルは胎盤の発達と機能の 重要な側面を正確に再現しています
- 胎盤の生物学と妊娠障害の研究のための 変容的なプラットフォームを提供します
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