液体 核 凝縮 は ゲノム を 機械 的 に 感知 し,再構成 する
Yongdae Shin1, Yi-Che Chang2, Daniel S W Lee3
1Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA; Department of Mechanical and Aerospace Engineering, Seoul National University, Seoul 08826, South Korea.
Cell
|December 1, 2018
まとめ
原子核における生物分子の液相分離は,クロマチンを機械的に排除し,より柔らかいユークロマティックな領域を好む. 標的DNAはコンデンサトの凝結によって引き合わされ,メカノアクティブフィルターとして作用します.
科学分野:
- 細胞生物学
- バイオ物理学
- ゲノミクス
背景:
- 細胞内組織は 生物分子液相移行に依存しています
- 本質的に無秩序なタンパク質 (IDP) の液体相分離が核体の形成を促す.
- クロマチンの構造に対するこれらの凝縮物の機械的影響は十分に理解されていません.
研究 の 目的:
- 液体コンデンサートからの物理的な力がクロマチンを再構成する方法を調査する.
- 凝縮物形成とクロマチンの組織における機械的性質の役割を探求する.
主な方法:
- CRISPR-Cas9ベースの光遺伝技術であるCasDropを使用しました
- 液体コンデンサに様々なIDPの相分離を観察した.
- 凝縮物の振る舞いを説明する 最小限の物理モデルを開発した.
主要な成果:
- IDPコンデンサは 成長するにつれて 機械的にクロマチンを排除します
- 凝縮物は,硬さ感受性のために,好ましくは低密度,無色領域で形成されます.
- 表面張力による凝結は 標的となるゲノム位置を 機械的に引き寄せます
結論:
- 核凝縮物はメカノ活性クロマチンフィルターとして作用する.
- これらのコンデンサートは,非標的領域を除外し,標的場所を集中することによって,物理的にクロマチンを操作します.
- 位相分離は,クロマチンに作用する力によって核の組織に影響を与えます.
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