人間のRNA結合タンパク質と,トランスレーション停止中のその動態
Jakob Trendel1, Thomas Schwarzl2, Rastislav Horos2
1German Cancer Research Center (DKFZ), Im Neuenheimer Feld 581, Heidelberg, Germany; European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, Heidelberg, Germany; Collaboration for joint PhD degree between EMBL and Heidelberg University, Faculty of Biosciences.
Cell
|December 12, 2018
まとめ
XRNAXは 相互リンクしたタンパク質-RNA複合体を 浄化する普遍的な方法です この技術は,様々な条件下でRNA結合プロテオームとタンパク質-RNA相互作用の包括的な分析を可能にします.
科学分野:
- 分子生物学
- 生物化学
- ゲノミクス
背景:
- タンパク質とRNAの相互作用は,多くの生物学的プロセスに不可欠です.
- 既存の方法は,タンパク質-RNA複合体を浄化する普遍性が欠けている.
- これらの相互作用を理解することは 細胞の機能を解読する鍵です
研究 の 目的:
- タンパク質とクロスリンクされたRNAの全般的な浄化のための新しい方法であるXRNAXを導入する.
- タンパク質とRNAの相互作用を研究するためのXRNAXの汎用性を実証する.
- RNA 結合タンパク質の組成と動態を特徴づける.
主な方法:
- XRNAX (クロスリンクされたRNA浄化) の方法の開発.
- 浄化された複合体を分析するために,トランスクリプトミクとプロテオミクのアプローチの適用.
- XRNAXとクロスリンクする免疫降水配列の統合 (CLIP-seq).
主要な成果:
- XRNAXは全てのRNAバイオタイプを成功裏に捕捉します
- コーディングRNAおよびノンコーディングRNA (ncRNA) と相互作用するサブプロテオームの特徴化.
- 数百のタンパク質-RNAインターフェースの識別と,ストレス下でのRNA結合プロテオームのダイナミックな改造.
- XRNAX-CLIP-seqを用いたラミンB1とEXOSC2とのncRNA相互作用の検証
結論:
- XRNAXは,タンパク質とRNAの相互作用を研究するための多用途で巧妙なアプローチを提供します.
- この方法は,RNA生物学の構造的および構成的側面のより深い理解を容易にする.
- XRNAXはRNA-タンパク質複合体の動的変化の研究を可能にします.
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