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関連する概念動画

Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

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At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
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Confocal Fluorescence Microscopy01:16

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Total Internal Reflection Fluorescence Microscopy01:05

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

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Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
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Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
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生細胞超解像度顕微鏡用の小分子光探査機

Lu Wang1, Michelle S Frei1,2, Aleksandar Salim1,2

  • 1Department of Chemical Biology , Max Planck Institute for Medical Research , Jahnstrasse 29 , 69120 Heidelberg , Germany.

Journal of the American Chemical Society
|December 15, 2018
PubMed
まとめ

小分子光探査機は,活体細胞の超高解像度顕微鏡で,動的細胞のプロセスを可視化するのに不可欠です. 探査機の開発における課題を克服することは この強力なイメージング技術を 進歩させるための鍵です

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科学分野:

  • 細胞および分子画像
  • バイオ物理学
  • 化学生物学

背景:

  • 超解像度の光顕微鏡は,バイオ分子と細胞構造のナノスケール可視化を提供します.
  • これらの技術で生細胞を画像化することで,ダイナミックな生物学的プロセスを高解像度で研究することができます.
  • 主な制限は,特定のバイオ分子にラベルを付けるのに適した光探知器の欠如です.

研究 の 目的:

  • 活細胞超解像度顕微鏡における小分子光探知器の重要性について議論する.
  • このアプリケーションに有効なプローブを作成する際の課題を強調します.
  • ラベリング戦略と探査特性における最近の進歩をレビューする.

主な方法:

  • 小分子光プローブに関する現在の文献のレビュー.
  • 生細胞画像探査機の化学的およびスペクトル学的要件の分析.
  • ラベル付け戦略と探査機の性能への影響についての議論

主要な成果:

  • 小分子探査機は,生細胞超解像度顕微鏡の現在の限界を克服するために不可欠です.
  • 開発には,特定の化学的,スペクトル学的課題に取り組む必要があります.
  • 新興のトレンドは,ラベリング戦略とプローブ設計の改善に焦点を当てています.

結論:

  • 小分子光探査機は,生細胞の超高解像度顕微鏡の進歩において重要な役割を果たしています.
  • これらの画像技術の可能性を最大限に活用するには,探査機の開発における継続的なイノベーションが必要である.
  • この展望は,探査機の設計と応用における将来の研究分野を概説しています.