2 細胞マウス胚における LincGETバイアスの細胞運命を非対称的に表現する
Jiaqiang Wang1, Leyun Wang2, Guihai Feng2
1State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101 Beijing, China; University of Chinese Academy of Sciences, 100049 Beijing, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, 100101 Beijing, China; College of Life Science, Northeast Agricultural University, 150030 Harbin, China.
Cell
|December 15, 2018
まとめ
哺乳類の胚の初期レギュレータとして機能する,新しい長いノンコーディングRNAであるLincGET. CARM1と相互作用し,遺伝子発現とクロマチンのアクセシビリティに影響を与えることで,細胞の運命を内部の細胞量に向ける.
科学分野:
- 発達生物学
- エピジェネティクス
- ゲノミクス
背景:
- 初期の哺乳類の胚の細胞運命を決定する初期段階は 発達に不可欠ですが まだ十分に理解されていません
- 早期の細胞運命を 分離させるメカニズムを理解することは 胚の発達を解読する鍵です
研究 の 目的:
- 哺乳類の胚における早期の細胞運命バイアスの確立における長いノンコーディングRNAの役割を調査する.
- 細胞内質量 (ICM) 系統の新規調節体を特定する.
主な方法:
- 2〜4細胞のマウス胚における長い非コーディングRNA LincGETの発現分析.
- リンカゲットとCARM1の過剰発現と枯渇を伴う機能研究
- 染色体免疫降水と遺伝子発現分析により,表層遺伝的変異と遺伝子活性化が評価される.
主要な成果:
- LincGETは初期のマウス胚で一時的に非対称的に発現します.
- LincGETの過剰発現は,ICMの運命に向かってブラストメアの子孫をバイアスする.
- LincGETはCARM1と相互作用し,H3R26me,ICM遺伝子発現,トランポゾンアップレギュレーション,およびクロマチンアクセシビリティを促進する.
結論:
- LincGETは哺乳類の胚の細胞運命を早期に調節するものである.
- ICMの運命に対する LincGETの系統バイアス効果は,CARM1に依存しています.
- この研究は 初期の細胞運命を制御する 新種の表遺伝的メカニズムを明らかにしています
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