全巻:単一の酵素ナノ粒子内の酵素の安定化

Robert Chapman¹, Martina H Stenzel¹

¹Centre for Advanced Macromolecular Design (CAMD), School of Chemistry , University of New South Wales , Sydney , New South Wales 2052 , Australia.

Journal of the American Chemical Society

|January 10, 2019

まとめ

単一の酵素ナノ粒子 (SENs) は,酵素の安定性と調整可能な活性性を向上させます. この革新的なアプローチは酵素を厳しい環境から保護し触媒の寿命を延長しオンデマンドの活性制御を可能にします

科学分野:

バイオテクノロジー
材料科学
酵素工学

背景:

酵素は産業や製薬に不可欠な触媒ですが,その安定性はしばしば制限されています.
酵素の長寿を向上させることを目指しています酵素の長寿を向上させることを目指しています
酵素の活性,安定性,調整可能な制御のバランスをとることは依然として重要な課題です.

研究の目的:

酵素の安定性と活性制御の強化のための単一の酵素ナノ粒子 (SENs) の可能性を調査する.
SEN のポリマーシェルが,要求の高い環境における酵素性能にどのように影響するか調査する.
先進生物触媒の有望なプラットフォームとしてSENを強調する.

主な方法:

酵素はポリマーの殻を使って個々のナノ粒子の中に封じ込められています
製造方法には,インサイトポリメリゼーションまたは酵素の周りにプリフォームされたポリマーの組み立てが含まれます.
特徴付けは,酵素活性,安定性,および基質拡散制御に焦点を当てています.

主要な成果:

SENは驚くべき安定性を示し,敏感な酵素でも高い初期活性を維持します.
ポリマーの殻は,溶媒,温度,pHによる変性から保護します.
酵素活性は,殻の材料の性質を調整することによって効果的に微調整することができます.

結論:

SENはバイオカタリシスの大きな可能性を持つ新興分野です.
この技術は,厳しい条件下で酵素の触媒寿命を延長します.
SENは,外部調節による酵素活動の可逆的なオン/オフを可能にします.

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