関連する実験動画
Updated: Jan 31, 2026

08:31
Antibody-Free Assay for RNA Methyltransferase Activity Analysis
Published on: July 9, 2019
7.6K
SETD3はアクチンヒスティジンメチルトランスフェラーゼで,プライマリディストシアを予防する
Alex W Wilkinson1, Jonathan Diep2, Shaobo Dai3
1Department of Biology, Stanford University, Stanford, CA, USA.
Nature
|January 11, 2019
まとめ
科学者たちは,ヒスティジン73でアクチンをメチル化する酵素として,SET領域タンパク質3 (SETD3) を特定した. このアクチン変異は滑らかな筋肉の機能とマウスの生殖能力に不可欠です.
科学分野:
- 生物化学
- 分子生物学
- 細胞生物学
背景:
- ヒスティジン73 (His73) で哺乳類のアクチンのメチル化は50年以上前から認識されている.
- 種間での普及と保存にもかかわらず,His73メチル化と責任ある酵素の機能は不明のままでした.
研究 の 目的:
- アクチンHis73メチル化酵素を特定する
- アクチンダイナミクスと細胞プロセスに対するHis73メチル化の機能的影響を解明する.
- SETD3とアクチンHis73の in vivo メチル化の生理学的役割を調査する.
主な方法:
- SETD3-アクチンの相互作用を視覚化するために,タンパク質構造の決定 (X線結晶学).
- アクチンヌクレオチド交換とフィラメント組成に対するHis73メチル化の影響を評価する生化学的試験.
- SETD3欠乏したマウスの生成と分析
- SETD3基質を特定するための定量プロテオミクス
- 人間の子宮の滑らかな筋肉細胞のインビトロ研究
主要な成果:
- SETドメインタンパク質3 (SETD3) は,アクチンHis73の特異的なメチルトランスファーゼとして特定されました.
- 構造分析により,SETD3の触媒部位内にアクチンHis73を定着させる正確なメカニズムが明らかになった.
- His73 メチル化によりアクチンモノメールの核酸交換が減少し,フィラメントの組み立てが促進することが示された.
- SETD3欠乏したマウスは,アクチンHis73メチル化が全くなく,生殖能力が低下した (母性ダイストキア).
- SETD3は,生理学的なアクチンHis73メチル化に責任を負う唯一の酵素であることが確認された.
- SETD3の減少は,ヒト子宮の滑らかな筋肉細胞の信号誘発収縮を危うくしました.
結論:
- SETD3は,アクチンHis73メチル化に責任を持つ哺乳類ヒスティジンメチルトランスフェラーゼである.
- アクチンHis73のメチル化はアクチンダイナミクスを調節し,滑らかな筋肉の収縮性と女性の生殖成功に不可欠です.
- タンパク質ヒスティジンメチル化は,細胞機能における重要な,しかし過小評価されている,規制メカニズムを表しています.
関連する概念動画
Actin Treadmilling
9.7K
Actin filaments undergo polymerization and depolymerization from either end. The polymerization and depolymerization rates depend on the cytosolic concentration of free G-actins. The polymerization rate is generally higher at the plus or barbed end, while the depolymerization rate is higher at the minus or pointed end. At a steady state, critical concentration describes the concentration of free G-actin monomers at which the polymerization rate at the plus end is equal to that of the...
9.7K
Cancer Prevention
8.1K
Several factors can increase the risk of cancer in an individual. About 50% of cancer cases can be prevented by adopting a healthy lifestyle, regular exercise, eating healthy, and following a modest cancer prevention diet. Epidemiological studies have consistently shown that populations with vegetable and fruit-rich diets have reduced the incidence of cancer. On the other hand, populations who have a diet rich in animal fat, red meat, junk food, or high calories are predisposed to cancer.
Some...
Some...
8.1K
Introduction to Actin
6.6K
Actin is a highly conserved cytoskeletal protein found abundantly in eukaryotic cells. It constitutes 10% weight of the total cellular protein in muscle cells, while in non-muscle cells, it is lower and makes up around 1–5 percent of the total cell protein. Actin found in the unicellular amoebae and complex multicellular animals is around 80% similar, demonstrating their conservation over a billion years of evolution. Actin coding genes are conserved within species and across...
6.6K
Actin Polymerization
8.6K
Actin polymerization occurs through the head-to-tail association of binding sites on monomeric actin or G-actin to form filamentous or F-actin. The polymerization can be divided into three phases ̶ nucleation, elongation, and steady-state phase.
The nucleation phase involves forming a stable nucleus consisting of three actin monomers to form a new actin filament. Actin-binding proteins such as formins and Arp2/3 complex help filament growth post-nucleation. The Formins form straight...
The nucleation phase involves forming a stable nucleus consisting of three actin monomers to form a new actin filament. Actin-binding proteins such as formins and Arp2/3 complex help filament growth post-nucleation. The Formins form straight...
8.6K
Prevention of Further Absorption of Poison
1.3K
In cases of acute poisoning, the primary objective is to prevent further absorption of the toxic substance into the body. Immediate interventions using various decontamination techniques targeting the gastrointestinal (GI) tract can achieve this. Decontamination is crucial to prevent poison from entering the systemic circulation, which involves washing affected areas with water and mild soap and removing contaminated clothing. Once external decontamination is done, attention must be turned to...
1.3K
Preventive Healthcare Services
2.2K
Preventive healthcare services keep people healthy via frequent check-ups, screening, and counseling. They primarily aid in disease prevention rather than treating an acute or chronic illness. Preventive treatment also keeps individuals productive and energetic, allowing them to work well into their retirement years. Examples of preventive care services include:
2.2K

