モバイル PEAR トランスクリプションファクターは,ポジショナルのシグナルをプライム カンビアル 成長に統合する
Shunsuke Miyashima1,2, Pawel Roszak1,3, Iris Sevilem1
1Institute of Biotechnology, HiLIFE/Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, Viikki Plant Science Centre, University of Helsinki, Helsinki, Finland.
Nature
|January 11, 2019
まとめ
植物の放射的成長は,成長のための遺伝子を活性化し,HD-ZIP IIIタンパク質と境界を形成するPEARタンパク質によって組織されます. このネットワークは 根の発達のためのホルモンとmiRNA信号を統合します
科学分野:
- 植物生物学
- 発達生物学
- 遺伝学
背景:
- 植物の放射性成長は発達に不可欠ですが,その調節は十分に理解されていません.
- このプロセスは,プロカンビウム細胞の初期プリミングと,後に血管カンビウムによる活性化を含みます.
- 動物のシステムにおける 発達パターンと類似点があります
研究 の 目的:
- アラビドプシスの根で放射的成長を開始する分子メカニズムを解明する.
- 細胞分裂と成長の境界を定めるのに関与する重要な調節体を特定する.
主な方法:
- アラビドプシスの根のプロカンビア組織における遺伝子発現パターンを調査した.
- PHLOEM EARLY DOF (PEAR) タンパク質とHD-ZIP III転写因子の機能を分析した.
- 遺伝子発現の調節におけるサイトキニン,オクシン,およびマイクロRNA (miR165/166) の役割を研究した.
主要な成果:
- サイトキニンのシグナル伝達により,プロトフローームのシート要素のPEARタンパク質 (PEAR1,PEAR2,DOF6,TMO6,OBP2,HCA2) が誘発される.
- PEARタンパク質は,放射性成長遺伝子を活性化するグラデントを形成します.
- オクシンとmiRNAによって調節されるHD-ZIP IIIタンパク質は,PEARタンパク質と対抗し,フィードバックループと分裂境界を形成する.
結論:
- PEARとHD-ZIP IIIの転写因子を含む規制ネットワークは,根の放射的成長を制御する.
- このネットワークは空間的なホルモンとmiRNA情報を統合し,分裂する細胞と静止する細胞の隣接するゾーンを確立します.
- このメカニズムは 植物における 持続的な放射的成長の基盤を 提供します
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