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研究分野エンジニアリング化学工学粉末と粒子の技術
CRISPR-Cas9 循環型パーミュータントは,ゲノム改変のためのプログラム可能な基板です.

CRISPR-Cas9 循環型パーミュータントは,ゲノム改変のためのプログラム可能な基板です.

Benjamin L Oakes ¹, Christof Fellmann ², Harneet Rishi ³, Kian L Taylor ⁴, Shawn M Ren ⁵, Dana C Nadler ⁵, Rayka Yokoo ⁵, Adam P Arkin ⁶, Jennifer A Doudna ⁷, David F Savage ⁵

Benjamin L Oakes ¹, Christof Fellmann ², Harneet Rishi ³

¹Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA.
²Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Gladstone Institutes, San Francisco, CA 94158, USA.
³Graduate Group in Biophysics, University of California, Berkeley, Berkeley, CA 94720, USA.
⁴Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA.
⁵Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
⁶Department of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
⁷Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Gladstone Institutes, San Francisco, CA 94158, USA; Graduate Group in Biophysics, University of California, Berkeley, Berkeley, CA 94720, USA; Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA.

⁰Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA.

Cell +

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2019年1月12日

関連する実験動画

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Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Published on: November 3, 2020

Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish

Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish

Published on: October 27, 2019

See all related videos

PubMedで要約を見る

まとめ

この要約は機械生成です。

円形のCas9タンパク質は先進的なゲノム工学ツールを提供しますこの新しい構造は精密な遺伝子編集と生物学的研究と医学のためのプロテアゼセンシングシステムの作成を可能にします

科学分野

生物化学
分子生物学
合成生物学

背景

CRISPR-Cas9は強力なゲノム編集ツールですがタンパク質融合と細胞活性化には限界があります
自然タンパク質の設計は将来の生物学的応用に不可欠です

研究の目的

再編成されたCRISPR-Cas9基板を使用して,RNAによるゲノム改変と保護のための高度なプラットフォームを開発する.
プログラム可能なインプットとアウトプットで細胞活動を感知する新型の単一分子エフェクタを作成します

主な方法

Cas9タンパク質の円形変異により,新しいトポロジカルな配列が作られます.
結合DNAに対するタンパク質末端の位置を体系的に調べる.
プロテアゼ感受性Cas9s (ProCas9s) の開発と特徴づけ

主要な成果

円形のCas9は戦略的な機能領域融合のためのプラットフォームを提供する.
ProCas9は,様々なプロテアスを感知できる単一分子エフェクタとして開発されました.
ProCas9は,病原体関連プロテアース活性に対する細胞反応を誘発する能力を示した.

結論

Cas9の循環変異はより安全で効率的なゲノム改変酵素プラットフォームを提供します.
この技術は分子レコーダーと精密ゲノム工学を研究,農業,生物医学で進歩させています

キーワード:

CRISPR-Casについてカス9-CP プロキャス9 円形変数融合タンパク質ゲノム編集タンパク質工学

関連する実験動画

自動的に一致したビデオです Contact us もしそれらが関連していない場合

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Published on: November 3, 2020

Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish

Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish

Published on: October 27, 2019

See all related videos

関連する概念動画

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

Histone Modification

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...

Genomics

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...

Spreading of Chromatin Modifications

Spreading of Chromatin Modifications

The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...

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