ヘテロキラル・コイル・コイル・フォーメーションにはヘンデカッド・モチーフが好ましい
Dale F Kreitler1, Zhihui Yao2, Jay D Steinkruger3
1Department of Chemistry , University of Wisconsin-Madison , Madison , Wisconsin 53706 , United States.
Journal of the American Chemical Society
|January 16, 2019
まとめ
l-およびd-ポリペプチドによって形成されるヘテロキラル・コイルド・コイルは,11-残留ヒンデカドの繰り返しを好みます. この発見により,ポリペプチドの相互作用とコイル・コイル設計の理解が進んでいます.
科学分野:
- 構造生物学
- 生物化学
- タンパク質科学
背景:
- コイル・コイル・アセンブリは,タンパク質構造において一般的です.
- L-ペプチドとd-ペプチドの相互作用は十分に理解されていません.
- 以前の研究では,M2-TMペプチドからヘテロキラルコイルドコイルドジマーを特定した.
研究 の 目的:
- ヘテロキラル・コイルド・コイルにおけるシーケンス・リピート・プレファレンスを調査する.
- ヘンデカッドモチーフが対極ヘリックスペアリングに内在するかどうかを判断する.
- サイドチェーンの改変がコイル・コイル・パッキングに与える影響を調べる.
主な方法:
- 3つのM2-TMペプチド変異体の結晶化
- 高解像度のX線結晶撮影
- 螺旋周波数と回転回転半径の分析
主要な成果:
- 3つの変種すべてにおいてヘテロキラル・コイル・コイル関連が観察された.
- 11-残留 (hendecad) シーケンスのモチーフを好むことを確認しました.
- 小さいサイドチェーンがコイル・コイル・パッキングを改善することを実証しました.
結論:
- ヘンデカドモチーフはヘテロキラスのコイル・コイル形成に内在する.
- サイドチェーンのエンジニアリングは,コイル・コイル・パッキングを最適化できます.
- 新しいヘテロキラル・コイル・コイル構造の設計のための基礎を提供する.
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