キウェリンは,分泌された真菌の毒性因子の代謝活動を無効化します
Xiaowei Han1, Florian Altegoer2, Wieland Steinchen2
1Department of Organismic Interactions, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.
Nature
|January 18, 2019
まとめ
トウモロコシキウェリンZmKWL1は,ウスティラゴ・メイディスの毒性因子である真菌酵素Cmu1を抑制する. この発見により 菌類の病原体に対する 新しい植物防御機構が明らかになり 植物免疫における キウェリンの役割が 強調されました
科学分野:
- 植物病理学
- 分子生物学
- 生物化学
背景:
- 菌根性植物病は 世界的な食糧安全保障を脅かしています
- ウスティラゴ・メイディス (Ustilago maydis) は,毒性のエフェクターを分泌することで,トウモロコシの汚れ病を引き起こす.
- 菌類のコリスマート変異酵素Cmu1は植物免疫信号伝達に干渉する.
研究 の 目的:
- 菌類の毒性を抑制するトウモロコシのタンパク質を特定する
- トウモロコシキウェリンとCmu1の相互作用のメカニズムを解明する.
- 植物防御におけるキウェリンの役割を探求する.
主な方法:
- トウモロコシキウェリンのCmu1阻害をテストする生化学的測定法
- ZmKWL1-Cmu1の相互作用の構造分析
- 植物キウェリンの遺伝子解析
主要な成果:
- トウモロコシキウェリンZmKWL1は,特に真菌Cmu1の触媒活性を抑制する.
- ZmKWL1は特定の相互作用によってCmu1の活性部位をブロックする.
- 系統遺伝学的分析は,キウェリンが病原体エフェクターに対抗する多用途であることを示しています.
結論:
- ZmKWL1はUstilago maydisに対する新しい植物防御機構を表しています.
- 植物キウェリンは,病原体エフェクター中和のための多用途の支架を持っています.
- この研究は,キウェリンの植物免疫における,以前未知の生物学的役割を明らかにした.
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