タンパク質の細胞内配送のためのDNA機能化された金属有機枠ナノ粒子

Shunzhi Wang¹, Yijing Chen¹, Shuya Wang²

¹Department of Chemistry and the International Institute for Nanotechnology , Northwestern University , 2145 Sheridan Road , Evanston , Illinois 60208 , United States.

Journal of the American Chemical Society

|January 24, 2019

まとめ

科学者は新しいナノ粒子のタンパク質の媒体を開発し細胞の吸収を10倍にしましたこの突破は診断と治療のためのタンパク質の供給を容易にするのです

科学分野:

バイオテクノロジー
ナノテクノロジー
材料科学

背景:

タンパク質は大きさと敏感性のために細胞に届くのは困難です
既存のタンパク質供給方法は,診断と治療の用途ではしばしば非効率です.

研究の目的:

効率的なタンパク質配送のための新しい金属有機枠ナノ粒子 (MOF NP) を設計し合成する.
これらのMOF NP-タンパク質結合体の構造的性質と細胞の吸収を調査する.

主な方法:

合成されたMOFNP (NU-1000,PCN-222/MOF-545) が,リン酸末端オリゴヌクレオチドと結合している.
特徴的なMOF NP構造とコロイドの安定性
インスリンをモデルタンパク質として使って,タンパク質を積んだMOFNPの細胞吸収を評価した.

主要な成果:

MOFNPはタンパク質を効果的にホストし,オリゴヌクレオチドに富んだ表面が安定性と細胞への侵入を保証します.
高いタンパク質の負荷能力を達成した.
本来のインスリンと比較して細胞の吸収を10倍に増やすことが示されました

結論:

哺乳類の細胞にタンパク質を供給する新しいMOFNPプラットフォームを開発しました.
このアプローチは様々なタンパク質を診断用プローブや治療薬として提供する可能性を示しています
MOFNPのオリゴヌクレオチド機能化は,細胞転移を促進する鍵です.

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