酵素による脱ガスされた表面誘発型原子移転分子ポリメリゼーションとリアルタイムモニタリング
Luis A Navarro1, Alan E Enciso2, Krzysztof Matyjaszewski2
1Department of Mechanical Engineering and Materials Science , Duke University , 101 Science Drive , Durham , North Carolina 27708 , United States.
Journal of the American Chemical Society
|January 25, 2019
まとめ
グルコース酸化酵素 (GOx) は,空気中の表面誘発の基質ポリメリゼーションを可能にします. GOxアシストドアトムトランスファーラジカルポリメリゼーション (ATRP) は,ポリマーブラシの成長運動を改善し,より多くのタンパク質を排斥して,アンチファウリング特性を強化します.
科学分野:
- ポリマー化学
- 表面科学
- バイオ材料工学
背景:
- ポリマーブラシのコーティングにはラジカルポリメリゼーションが不可欠ですが,酸素に非常に敏感です.
- 酵素による酸素スキャビングは制御されたポリメリゼーションのための潜在的な解決策を提供します.
- グルコース酸化酵素 (GOx) は酸素を消費し,有酸素状態でのポリメリゼーションを容易にする.
研究 の 目的:
- 周囲の空気中の表面発起ポリマーブラッシュの増殖を助けるために,グルコース酸化酵素 (GOx) の使用を調査する.
- ポリマー化運動とポリマーブラシの特性に対するGOxの影響を評価する.
- GOx改変ポリマーブラシコーティングの防腐性能を評価する.
主な方法:
- 生物医学的に重要なポリマー (POEGMA,PDMAEMA,PSBMA,PMSEA) の表面誘発ATRPは,GOxの存在または存在しない状態にある.
- クォーツ結晶マイクロバランス (QCM) と局所表面プラズモン共鳴 (LSPR) を使用して,ポリメリゼーション運動のリアルタイムモニタリング.
- 反応パラメータを分析するために,二分子終結を含むブラッシュの成長の運動モデリング.
主要な成果:
- GOxで支援されたATRPは,空気にさらされた表面からポリマーブラシを成功裏に生産しました.
- GOxは,ポリマーブラッシュの成長の有効な運動連鎖長,増殖率,再現性を有意に増加させた (R2 > 0. 987).
- GOxで作られたポリマーコーティングは,GOxのない対照と比較して,より多くのヒト血タンパク質を排斥する優れた防腐性能を示しました.
結論:
- GOxは,露天での表面発射ATRPを有効にするために有効で安価な添加物です.
- GOxによるATRPは,ポリマーブラシの成長の制御と,防腐性の強化につながります.
- この酵素学的アプローチは,性能を向上した先進的なバイオマテリアルを開発するための有望な戦略です.
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