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局所性前立腺がんにおける広範囲および機能的RNA循環
Sujun Chen1, Vincent Huang2, Xin Xu3
1Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada; Princess Margaret Cancer Center, University Health Network, Toronto, Ontario, Canada; Ontario Institute for Cancer Research, Toronto, Ontario, Canada.
Cell
|February 9, 2019
まとめ
前立腺がんの超深層RNAシーケンシングは,異なったトランスクリプトミックのサブタイプと広範囲にわたる円形RNA生成を明らかにします. これらの円形RNAは癌細胞の増殖に不可欠であり,疾患の進行と関連しています.
科学分野:
- 腫瘍学
- ゲノミクス
- 分子生物学
背景:
- 癌のトランスクリプトームは複雑で,少量の非ポリアデニル化トランスクリプトを含んでいます.
- 局所性前立腺がんのトランスクリプトームの包括的な特徴づけが必要である.
研究 の 目的:
- 局所性前立腺がんに 超深層の全RNA配列解析を行うこと
- 病気の特徴に関連したトランスクリプトミックのサブタイプと分子特性を特定する.
- 前立腺がんの進行と増殖における円形RNAの役割を調査する.
主な方法:
- 局所的な前立腺腫瘍144件でポリA選択なしの超深層全RNA配列解析 (RNA-seq) を実施した.
- バックスプライシングイベントを含む線形および円形RNA発現の分析.
- 細胞増殖のための円形RNAの必要性を評価するための機能喪失スクリーニング.
主要な成果:
- 線形トランスクリプトミックのサブタイプが 特定されました 線形トランスクリプトミックのサブタイプが 特定されました 線形トランスクリプトミックのサブタイプが 特定されました
- 転移した前立腺がんと 異なる融合プロファイルを発見した
- 広範囲に広がる円形RNA (circRNA) 産生 (平均7232個/腫瘍),疾患の進行と相関する.
- 豊富な circRNAの11.3%が細胞増殖に不可欠であり,明確な機能を持つことが判明しました.
結論:
- ポリA選択なしの超深層RNA-seqは,包括的なトランスクリプトーム分析に不可欠です.
- 円形のRNAは前立腺がん細胞の増殖と進行に重要な役割を果たします.
- circRNAは前立腺がんの潜在的治療標的とバイオマーカーです.
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