ミトコンドリア,エンドプラズマ網膜,およびライソソームの膜張りのイメージに機械的に敏感な光探査機

Antoine Goujon¹, Adai Colom¹, Karolína Straková¹

¹School of Chemistry and Biochemistry and ‡National Centre of Competence in Research (NCCR) Chemical Biology , University of Geneva , CH-1211 Geneva , Switzerland.

Journal of the American Chemical Society

|February 13, 2019

まとめ

新しい光探知器は細胞の臓器内の膜の緊張を測定しますこれらの探査機はライソソーム,ミトコンドリア,エンドプラズマ網膜を標的とし,オルガネルの機能とダイナミクスに関する新しい洞察を可能にします.

科学分野:

細胞生物学
バイオ物理学
分子イメージング

背景:

細胞内の力を測定するのは難しい.
力の測定には光探知器が必要である.
以前の探査は,光生涯イメージング顕微鏡 (FLIM) を使用して,プラズマ膜の緊張を標的とした.

研究の目的:

細胞内オーガネルの張力測定のための新しいフリッパープローブを設計,合成し,評価する.
リソソーム,ミトコンドリア,エンドプラズマ網膜などの特定の臓器内で緊張画像を撮影します

主な方法:

オーガネル特異的なフリッパープローブの開発と合成.
張力測定のための光生涯画像顕微鏡 (FLIM) を利用する.
膜張りの変化を誘導する光ショックを適用する

主要な成果:

フリッパー探知器は選択的にライソソーム,ミトコンドリア,エンドプラズマ網を標識する.
膜の緊張の変化に対する感受性を確認した.
臓器細胞特有の光寿命は,静止状態の緊張ではなく,膜組織の違いを示した.
器官のスケールでの生命期の異質性は,ER管,シート,核膜の洞察をもたらした.

結論:

細胞内を標的とする光器の探知器は臓器の機能に前例のない洞察力を提供します
これらの探査機は,細胞生物学において以前はアクセス不可能な問題,例えばエンドソームの密輸やミトコンドリアのダイナミクスに対処できる.

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