2つの酵素経路は,N-ニトロゾの生物合成において,l-アルギニンを酸化窒素と結びつける
Hai-Yan He1, Alyssa C Henderson1, Yi-Ling Du2
1Department of Chemistry , University of British Columbia , Vancouver , Canada.
Journal of the American Chemical Society
|February 15, 2019
まとめ
科学者は,ストレプトゾシン生物合成中にl-アルギニンから窒素酸化物 (NO) の生成のための新しい経路を発見しました. この研究は,NO生成とN-ニトロゾ化合物の形成に関与する新しい酵素を明らかにした.
科学分野:
- 生物化学
- 自然製品の生物合成
- 酵素学
背景:
- 窒素酸化物 (NO) は様々な生物学的役割を果たしますが,化学的多様性の発生への関与はあまり理解されていません.
- N-ニトロゾ化合物は,重要な生物学的意味を持つ分子の一種である.
研究 の 目的:
- N-ニトロゾシン化合物の生物合成経路を解明する.
- この経路におけるN-ニトロゾ群の発生源とNO形成のメカニズムを特定する.
主な方法:
- ストレプトゾシン生物合成遺伝子群の特定
- StzE (N-メチル化) とStzF (酸化) を特徴付ける酵素分析
- NOと尿素化合物を含む反応産物の分析
主要な成果:
- ストレプトゾシンのN-ニトロゾ群は,窒素-窒素結合形成によるl-アルギニンから発生する.
- StzEはl-アルギニンをNω-モノメチル-l-アルギニンにメチル化する.
- StzFは,新しい非ヘム鉄依存酵素で,Nω-モノメチル-l-アルギニンを酸化して,NOと尿素化合物を生成する.
結論:
- 単一の酵素ペア (StzEとStzF) を含む,L-アルギニンからNOの生成のための新しい酵素経路が発見されました.
- この発見は,生体内でのN-ニトロゾ化合物の形成は,NOの生成とその後の捕獲によって起こる可能性を示唆している.
- この研究は,N-ニトロゾ天然製品の生物合成を理解するための基礎を提供します.
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