レジスティヴ・パルス・ナノポー・センシングで観察されたチオラート・キャプテッド・ゴールド・ナノクラスターのリガンド誘発構造変化
Bobby D Cox1, Patrick H Woodworth1, Peter D Wilkerson1
1Department of Physics , Virginia Commonwealth University , Richmond , Virginia 23284 , United States.
Journal of the American Chemical Society
|February 19, 2019
まとめ
この研究では,アルファ-ヘモリシン (αHL) ナノポールを用いて金ナノクラスターを検出しています. 電気信号の変化は,リガンド質量と相関し,表面変化の特徴づけを可能にします.
科学分野:
- バイオ物理学
- ナノテクノロジー
- 分析化学
背景:
- ナノポーレセンシングは バイオポリマーを分析するものです
- 新興アプリケーションには,水溶性金属クラスタの特徴付けが含まれています.
- アルファ-ヘモリシン (αHL) は生物学的ナノ孔である.
研究 の 目的:
- チオラートで覆われた金ナノクラスターを検出するためのαHLナノポールの使用を調査する.
- 異なるキャピングリガンドがナノ孔信号に与える影響を分析する.
- 粒子内表面の変化を特徴づけるためのナノ孔センサーを研究する.
主な方法:
- αHLナノポールを用いた抵抗性パルスセンシング.
- 黄金のナノクラスターをp-mercaptobenzoic酸 (p-MBA),ティオプロニン (TP),およびチオラ化PEG7 (S-PEG7) で覆った検出
- 孔に閉じ込められた個々のナノクラスターの電流の変動とサブステートの分析.
主要な成果:
- 個々のゴールドナノクラスターは αHL孔内で明確な電流変動を示した.
- 観測された電流のステップは,キャピングリガンドの質量 (p-MBA,TP,S-PEG7) と相関する.
- リガンド質量は,ナノ孔信号特性のスケーリング要因であることが判明した.
結論:
- αHLを用いたナノ孔検出は,表面リガンドに基づいて金ナノクラスタを区別することができる.
- この技術は,金属ナノクラスタの粒子内表面改変を特徴づける可能性を示しています.
- このアプローチは,ナノ粒子表面化学を分析するための新しい方法を提供します.
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