TAZ活動を調節するメカニカル信号によって媒介される細胞の仕様における横向的阻害
Peng Xia1, Daniel Gütl1, Vanessa Zheden1
1Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria.
Cell
|February 19, 2019
まとめ
機械的な信号が TAZ活動を通して 細胞の運命を調節します ゼブラフィッシュのオオゲネシスは,デルタノッチ信号だけでなく,細胞圧縮が横向的阻害を駆動し,TAZ核の局所化と細胞の分化に影響を与えることを明らかにします.
科学分野:
- 発達生物学
- 細胞生物学
- 機械生物学
背景:
- 横向抑制は,通常デルタノッチシグナル伝達によって媒介される細胞運命の仕様には極めて重要です.
- 横方向阻害を制御する正確なメカニズムとシグナルモードは完全に理解されていません.
研究 の 目的:
- ゼブラフィッシュのオオゲネシス中の横向的な阻害における代替信号機構を調査する.
- 細胞運命を決定するメカニカル・フォースとTAZ活動の役割を調査する.
主な方法:
- ゼブラフィッシュの粒状細胞におけるTAZ核の局所化観察
- マイクロファイルの前駆体細胞 (MPC) の切除と抽出を含む実験操作.
- 野生型とTAZ型ゼブラフィッシュの葉っぱのTAZ活性分析
主要な成果:
- 核のTAZ濃度の上昇はオオサイト動物極の細胞で観察され,MPCは最も高い蓄積を示した.
- 成長するMPCによる機械的圧縮により TAZは隣接する細胞の核から排除された.
- 圧縮を緩和して核TAZを回復し,隣接する細胞のMPCの運命を再指定した.
- TAZの重要な役割を確認した.
結論:
- TAZ活性を制御する機械的信号によって媒介される新しい側面阻害モードが発見されました.
- このメカニズムは,細胞運命の仕様における正規のデルタノッチシグナリングの代替手段を提供します.
- 機械的なシグナルは,転写共活性化器の活動と細胞運命を決定する上で重要な役割を果たします.
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