哺乳類のオルガノゲーシスの単細胞転写図

Junyue Cao^1,2, Malte Spielmann¹, Xiaojie Qiu^1,2

¹Department of Genome Sciences, University of Washington, Seattle, WA, USA.

Nature

|February 22, 2019

まとめ

この研究では,マウスの臓器生成を単細胞解像度でマッピングし,包括的な細胞アトラスを作成します. 胚の発達中の詳細な転写ダイナミクスと発達軌道を明らかにしています.

科学分野:

発達生物学
ゲノミクス
コンピュータ生物学

背景:

哺乳類のオルガノゲネシスには細菌の層が複雑な胚に急速に変化することが含まれます
臓器形成の過程における転写調節を理解することは,発達生物学にとって極めて重要です.

研究の目的:

単細胞解像度でのマウス器官生成の転写ダイナミクスを調査する.
総合的な"マウス・オルガノゲネシス・セル・アトラス" (MOCA) を作成する.

主な方法:

トランスクリプトームをプロファイルするために単細胞結合インデックスが使用されました.
61匹のマウス胚 (妊娠9. 5~13. 5日) の約200万個の細胞のデータを分析した.
モノクル3ソフトウェアは,細胞の種類と軌道を識別するために使用されました.

主要な成果:

発達過程の全体的な見方を提供する"マウス・オルガノゲネシス・セル・アトラス" (MOCA) が作成された.
何百もの細胞タイプと 56の発達経路が特定されました
何千ものマーカー遺伝子が定義され,アピカル・エクトダーマル・リッジ,肢体・メゼンキーム,骨格筋などの特定の胚構造に焦点を当てた分析が行われました.

結論:

この研究は,哺乳類の臓器形成に関する前例のない単細胞解像度の洞察を提供します.
MOCAは胚の発達中の遺伝子発現のダイナミクスを理解するための貴重なリソースです.
詳細な細胞経路とマーカー遺伝子は発達生物学と再生医療の将来の研究の基盤となります

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