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種間S-ニトロシル化によるマイクロRNA機構の調節と開発
Puneet Seth1, Paishiun N Hsieh2, Suhib Jamal1
1Institute for Transformative Molecular Medicine and Department of Medicine, Case Western Reserve University School of Medicine and University Hospitals Cleveland Medical Center, Cleveland, OH 44106, USA.
Cell
|February 23, 2019
まとめ
腸内微生物群は窒素酸化物を生成し ホストタンパク質とマイクロRNAの機能を変化させます このバクテリアのシグナル伝達は C. elegans の遺伝子発現と発達に影響を与え 新しい伝達経路を明らかにします
科学分野:
- 微生物学
- 分子生物学
- 発達生物学
背景:
- ホストと微生物群の相互作用は 細胞機能にとって極めて重要です
- 微生物と宿主との種間コミュニケーションのメカニズムは ほとんど不明です
- 酸化窒素 (NO) は多様な生物学的役割を持つガス伝達物質である.
研究 の 目的:
- 微生物の分子が宿主細胞の機能に どう影響するか調べる
- 宿主タンパク質の改変における居住細菌からの酸化窒素の役割を解明する.
- 宿主遺伝子の発現と発達に対する微生物に依存したタンパク質S-ニトロシレーションの影響を理解する.
主な方法:
- モデル生物C.エレガンスを利用した.
- 居住するバクテリアによる窒素酸化物の生成を調査した.
- アルゴナウト (ALG-1) を含む宿主タンパク質のS-ニトロシル化分析
- 窒素酸化物生成とS-窒素化経路の操作
主要な成果:
- 微生物から派生した酸化窒素は,C. elegansの宿主タンパク質の広範なS-ニトロシレーションを誘導する.
- 哺乳類のAGO2に保存されているALG-1の微生物に依存するS-ニトロシレーションは,そのマイクロRNA媒介の遺伝子調節機能を変化させる.
- 窒素酸化物生成の除去またはALG- 1 S- 窒素化により,宿主の発達に重大な影響がもたらされた.
結論:
- 腸内微生物群は 宿主の転移後の環境を形成します
- 微生物のシグナリングはマイクロRNAの活動,遺伝子発現,宿主の発達を調節する.
- この研究は,ガス伝達物質とタンパク質のS-ニトロシル化を含む宿主-微生物群のコミュニケーションの新しいメカニズムを明らかにしています.
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