核細胞は,マウスのベータメジャーグロービン遺伝子に沿って,赤色素体および非赤色素体細胞に段階的に配置されます
Cell
|March 14, 1986
まとめ
核細胞はマウスのベータメジャーグロービン遺伝子に沿って正確に位置する. 活性細胞では,この相性核細胞配列は,遺伝子の5'端の近くで破壊され,異なる構造状態を示します.
科学分野:
- 分子生物学は分子生物学である.
- エピジェネティクス エピジェネティクス
- ゲノミクスゲノミクスとは
背景:
- 核子の位置づけは,遺伝子調節において極めて重要です.
- ネズミのベータメジャーグロービン遺伝子は,赤血球細胞における遺伝子活性化を研究するためのモデルとして機能しています.
研究 の 目的:
- ネズミのベータメジャーグロービン遺伝子に沿った核細胞の正確な位置と配置を決定する.
- 細胞の不活性状態と活性状態の間のニュクレオソームの位置づけの違いを調査する.
主な方法:
- 核細胞マッピングのために,化学的分裂反応剤メチジウムプロピル-EDTA-Fe ((II) (MPE-Fe ((II)) を利用しました.
- ネズミのL細胞 (ノンレトロイド) とネズミの赤血球白血病細胞 (赤血球白血球) の核細胞分布を分析した.
主要な成果:
- ネズミの不活性なL細胞では,核細胞は,グロービン遺伝子領域 (-3000〜+1500) にわたって正確に段階的に配置されています.
- 誘発性赤血球白血病細胞と誘発性赤血球白血病細胞の両方で,この段階化は維持されますが, -200 から +500 の位置の間に中断されます.
- 活性細胞内のこの核細胞欠乏領域は,MPE-Fe (II) 消化から保護され,過敏な部位に囲まれています.
結論:
- マウスベータメジャーグロービン遺伝子は,核細胞組織に基づく少なくとも2つの異なる構造状態に存在する.
- 不活性状態は,連続した配列の相性核細胞を特徴とする.
- 活性状態は,遺伝子の5'領域におけるこの配列の破壊によって特徴付けられます.
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