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関連する概念動画

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

13.4K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

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Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
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Interference and Diffraction02:18

Interference and Diffraction

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Interference is a characteristic phenomenon exhibited by waves. When two electromagnetic waves interact with their peaks and troughs coinciding, a resulting wave with enhanced amplitude is produced. This is known as constructive interference. In this case, the two waves interacting are in phase with each other.
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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Internal Energy02:00

Internal Energy

36.7K
The total of all possible kinds of energy present in a substance is called the internal energy (U), sometimes symbolized as E. Suppose a system with initial internal energy, Uinitial, undergoes a change in energy (transfer of work or heat), and the final internal energy of the system is Ufinal. Change in internal energy equals the difference between Ufinal and Uinitial.
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Protein-protein Interfaces02:04

Protein-protein Interfaces

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Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
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Updated: Jan 28, 2026

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
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Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

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マイクロスケールの凸なインターフェースでの全体的な内部反射と干渉による色付け

Amy E Goodling1, Sara Nagelberg2, Bryan Kaehr3

  • 1Department of Materials Science and Engineering, The Pennsylvania State University, University Park, PA, USA.

Nature
|March 1, 2019
PubMed
まとめ
この要約は機械生成です。

新しい光学メカニズムは 凸なインターフェイスで光の干渉を通して 虹彩の構造的な色を生成します この現象は単純な水滴で観察され,高度なアプリケーションに制御可能な色を生成します.

さらに関連する動画

Total Internal Reflection Absorption Spectroscopy TIRAS for the Detection of Solvated Electrons at a Plasma-liquid Interface
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Total Internal Reflection Absorption Spectroscopy TIRAS for the Detection of Solvated Electrons at a Plasma-liquid Interface

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Nanotopology of Cell Adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy VA-TIRFM
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Nanotopology of Cell Adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy VA-TIRFM

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関連する実験動画

Last Updated: Jan 28, 2026

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
06:43

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

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Total Internal Reflection Absorption Spectroscopy TIRAS for the Detection of Solvated Electrons at a Plasma-liquid Interface
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Total Internal Reflection Absorption Spectroscopy TIRAS for the Detection of Solvated Electrons at a Plasma-liquid Interface

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Nanotopology of Cell Adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy VA-TIRFM
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Nanotopology of Cell Adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy VA-TIRFM

Published on: October 2, 2012

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科学分野:

  • 光学とフォトニクス
  • 材料科学

背景:

  • 吸収,分散,干渉などの物理現象が色を生み出します
  • 球状のドロップルは散乱,屈折,干渉を示し,大気光学効果に貢献します.

研究 の 目的:

  • 有意な角スペクトル分離を持つ虹彩構造色を生成するための新しいメカニズムを記述する.
  • この現象の適用性を様々なシステムで実証し,理論的な予測を検証する.

主な方法:

  • コンケーブな光学界面での全体的な内部反射による光の干渉を調査した.
  • 水滴のような単純なシステムや 多相滴やパターンの表面を含む複雑なシステムで この現象を観察した.
  • 実験的観測と一致する理論的予測を開発した.

主要な成果:

  • 凸なインターフェイスの内部反射に基づく虹彩構造の新しいメカニズムを発見した.
  • 見える光の波長よりもはるかに大きいインターフェースで生成された輝かしい色パターンを示しました.
  • 多様なマイクロスケールシステムで 制御可能な構造色を 達成した.

結論:

  • 新しく特定された光学メカニズムは,大きな角のスペクトル分離を持つ虹彩構造色の作成方法を提供します.
  • この現象は単純なシステムでは容易に観察でき,複雑なシステムでは制御できます.
  • この発見は 機能的な材料,ディスプレイ,センサーに 応用できる可能性があります