神経細胞の細胞結合は,N-CAMとヘパリンのような分子との相互作用に依存しています

G J Cole, A Loewy, L Glaser

Nature

|April 3, 1986

まとめ

ヘパラン硫酸に結合するニューラル細胞粘着分子 (N-CAM) は,ニューラル発達中の細胞-細胞相互作用に不可欠です. この相互作用は,N-CAMによって媒介されます.

科学分野:

発達神経科学とは
細胞結合メカニズム細胞結合メカニズム
分子生物学は分子生物学である.

背景:

細胞と細胞の相互作用は,細胞の移動と軸索のターゲティングを含む神経の発達に不可欠です.
神経細胞粘着分子 (N-CAM) は,同性愛結合を通じてニューロン-ニューロン粘着を媒介する.
また,N-CAMは細胞基板の相互作用にも参加し,ヘパラン硫酸塩の結合を必要とします.

研究の目的:

ヘパラン硫酸塩のN-CAMへの結合が細胞間の結合に不可欠であるかどうかを調査する.
神経細胞粘着におけるN-CAMのヘパリン結合ドメインの役割を明らかにする.

主な方法:

粘着測定のために網膜探査細胞と網膜細胞単層を使用した.
ヘパラン硫酸塩の役割を評価するためにヘパリンとコンドロイチン硫酸を使用した.
N-CAMのホモフィル結合ドメインとヘパリン結合ドメインを標的にする単一クローン抗体を使用した.
細胞粘着に対するN-CAMヘパリン結合ドメインの効果を分離し,試験した.

主要な成果:

ヘパラン硫酸のアナログであるヘパリンは,網膜の細胞-細胞結合を著しく抑制しました.
コンドロイチン硫酸は細胞間の結合に影響を与えず,特異性を示した.
N-CAMのヘパリン結合ドメインに対するモノクローナル抗体は,細胞-細胞結合を阻害した.
単離されたヘパリン結合ドメインの断片も,細胞-細胞結合を阻害した.

結論:

ヘパラン硫酸塩のN-CAMへの結合は,神経発達における同性細胞細胞結合に不可欠である.
N-CAMのヘパリン結合ドメインは,これらの細胞間の相互作用を媒介する上で重要な役割を果たします.
これらの発見は,N-CAMが細胞-基板および細胞-細胞結合の両方において二重の役割を果たしていることを明らかにしています.

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