レドックス制御されたサイト固有のα2-6-シアリレーション
Na Lu1, Jinfeng Ye1, Jiansong Cheng2
1National Glycoengineering Research Center, State Key Laboratory of Microbial Technology , Shandong University , Qingdao 266237 , China.
Journal of the American Chemical Society
|March 8, 2019
まとめ
フォトバクテリア・ダムセラ α2-6-シアリルトランスフェラーゼ (Pd2,6ST) を用いた新しい酸化還元制御法により,正確なサイト固有のシアリレーションが可能である. この戦略は望ましくない部位を隠し,複雑なα2−6結合シアロシドの標的合成を可能にします.
科学分野:
- 生物化学
- 酵素学
- グライコ化学
背景:
- Photobacterium damselae (Pd2,6ST) のバクテリアのα2-6-シアリルトランスフェラーゼは,α2-6結合シアロシドを合成するために不可欠である.
- Pd2,6STの広範な基板特異性は,複数のギャラクトースまたはN-アセチルギャラクトサミン単位を持つ複雑な分子のサイト固有のシアリレーションに課題をもたらす.
研究 の 目的:
- Pd2,6STを用いたサイト固有のα2-6シアライレーションのための一般的な戦略を開発する.
- 複雑なグリカン合成におけるPd2,6STの基板の柔軟性の限界を克服する.
主な方法:
- 特定のギャラクトース単位をシアライレーションから遮断するために酵素による酸化.
- Pd2,6STの活性を望ましい部位に誘導する.
- サイト固有のα2-6シアリレーション
主要な成果:
- サイト固有のシアライレーションのための新しいリドックス制御戦略が確立された.
- 望ましくないシアライレーション部位は酵素酸化によって効果的にマスクされた.
- ターゲットガラクトーゼとN- アセチルガラクトサミンの単位でα2-6シアライレーションを正確に制御することができました.
結論:
- 開発されたレドックス制御法では,Pd2,6STによる正確なサイト固有のα2-6シアライレーションが可能である.
- この戦略は,複雑なα2−6結合シアロシドを合成する能力を著しく高めます.
- このアプローチは,ターゲットを絞ったグリカン工学と合成のための汎用的なツールを提供します.
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