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Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons09:21

Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons

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This protocol describes the cell culturing of human midbrain dopaminergic neurons, followed by immunological staining and the generation of neuronal phenotypic profiles from acquired microscopic high-content images allowing the identification of phenotypic variations due to genetic or chemical...
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Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression10:54

Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression

10.0K
In Parkinson's Disease (PD), Substantia Nigra (SNc) dopaminergic neurons degenerate, leading to motor dysfunction. Here we report a protocol for culturing ventral midbrain neurons from a mouse expressing eGFP driven by a Tyrosine Hydroxylase (TH) promoter sequence, harvesting individual fluorescent neurons from the cultures, and measuring their transcriptome using...
10.0K
Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains08:45

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

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The causes of degeneration of midbrain dopaminergic neurons during Parkinson’s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of...
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Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice09:35

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

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This protocol describes two different environmental manipulations and a concurrent brain infusion protocol to study environmentally-induced brain changes underlying adaptive behavior and brain repair in adult...
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Primary Culture of Mouse Dopaminergic Neurons11:58

Primary Culture of Mouse Dopaminergic Neurons

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Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various...
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Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

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Dopamine is distinctly regulated in the midbrain nuclei, which contain the cell bodies and dendrites of the dopamine neurons. Here we describe a dissection and sample-handling approach to maximize results, and thus conclusions and insights, on dopamine regulation in the midbrain nuclei of the substantia nigra (SN) and ventral tegmental area (VTA) in...
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関連する実験動画

Updated: Jan 8, 2026

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
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Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

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心不全におけるミトコンドリア機能障害

Anupam A Kumar1, Daniel P Kelly1, Julio A Chirinos1,2

  • 1From the University of Pennsylvania Perelman School of Medicine, Philadelphia (A.K., D.P.K., J.C.).

Circulation
|March 12, 2019
PubMed
まとめ
この要約は機械生成です。

ミトコンドリア機能障害は,保存されたエジェクション分数 (HFpEF) の心不全における運動不耐症に寄与する. ミトコンドリアを標的とした治療は症状を緩和し,HFpEF患者にとって有望な治療法である.

キーワード:
MRI について運動不耐性発射分子が保存された心不全ミトコンドリア機能

さらに関連する動画

Primary Culture of Mouse Dopaminergic Neurons
11:58

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Published on: September 8, 2014

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Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area
09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

Published on: August 10, 2012

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関連する実験動画

Last Updated: Jan 8, 2026

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
09:35

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

Published on: January 20, 2015

9.2K
Primary Culture of Mouse Dopaminergic Neurons
11:58

Primary Culture of Mouse Dopaminergic Neurons

Published on: September 8, 2014

39.1K
Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area
09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

Published on: August 10, 2012

26.4K

科学分野:

  • 心臓病科
  • ミトコンドリア生物学
  • 運動 生理学

背景:

  • 発射分子が保存された心不全 (HFpEF) は異質な症候群である.
  • 運動不耐性は,心臓および外周的要因に起因するHFpEFの主要な症状です.
  • ミトコンドリア機能障害は,酸素利用の低下とHFpEFにおける運動不耐性に関連しています.

研究 の 目的:

  • HFpEFの病理生理学におけるミトコンドリア生物学の役割を検討する.
  • 人間におけるミトコンドリア機能の評価方法について議論する.
  • HFpEFの治療標的としてミトコンドリアを調査する.

主な方法:

  • HFpEFにおけるミトコンドリア機能の文献レビュー
  • 人間のミトコンドリア機能を評価するための現在の方法の分析.
  • ミトコンドリア機能障害とHFpEFを関連付ける証拠の検討

主要な成果:

  • ミトコンドリアの異常は,HFpEFにおける酸素利用の低下と運動不耐症に大きく寄与する.
  • 臨床環境でミトコンドリア機能を評価するための様々な方法があります.
  • ミトコンドリア機能障害は,HFpEFの病理学における重要な要因である.

結論:

  • ミトコンドリア機能はHFpEFの重要な治療目標である.
  • ミトコンドリア機能を標的とした臨床試験は進行中であり,新しい治療法の開発に不可欠です.
  • ミトコンドリアエージェントに関するさらなる研究は,HFpEFの症状を改善する見込みです.