世界的なヒト腸内微生物の未培養ゲノムからの新しい洞察
Stephen Nayfach1,2, Zhou Jason Shi3,4, Rekha Seshadri5,6
1United States Department of Energy Joint Genome Institute, Walnut Creek, CA, USA. snayfach@lbl.gov.
Nature
|March 15, 2019
まとめ
研究者らはヒトの腸内メタゲノムから60,664のプロカリオットゲノム草案を再構築し,2,058の新種レベルの運用分類単位 (OTU) を特定した. これは腸内細菌の多様性と 病気の関連性に関する理解を 大きく広げています
科学分野:
- 微生物学
- ゲノミクス
- バイオ情報学
背景:
- 多くのヒトの腸内微生物は 培養の課題により 配列化されていないままです
- 現存するゲノムデータは 微生物多様性のほんの一部にすぎません
研究 の 目的:
- 培養されていないヒトのゲノムを再構築する
- 既知の腸内微生物の遺伝子多様性のカタログを拡大する.
- 健康や病気における新たな種と その潜在的な役割を特定する.
主な方法:
- 3,810 人の糞便のメタゲノムから 60,664 の草稿の復元.
- 2,058 種のレベルでの新しい運用分類単位 (OTU) の特定
- 疾患関連性を特定するための臨床腸内微生物群の研究のメタ解析
主要な成果:
- 配列化された腸内細菌の遺伝子多様性が50%増加した.
- 新たに特定されたOTUは,農村部からの個人で一般的です.
- 多くの疾患関連が新しいOTUと特定され,予測モデルが強化されました.
- 分析により 培養されていない種で ゲノム減少が確認され 生物合成経路が失われました
結論:
- この研究はヒトの腸内微生物群のゲノム参照カタログを大幅に拡張しています.
- 新しい微生物種が発見され 腸の健康と病気の理解に 新たな目標が提供されています
- ゲノム減少に関する洞察は,未培養の微生物の将来の培養戦略を導くかもしれません.
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