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In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

7.0K
Here we describe a rapid and direct in vivo CRISPR/Cas9 screening methodology using ultrasound-guided in utero embryonic lentiviral injections to simultaneously assess functions of several genes in the skin and oral cavity of immunocompetent...
7.0K
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

7.5K
This protocol outlines the steps needed to generate a model system in which the transcription of an endogenous gene of interest can be conditionally controlled in live animals or cells using enhanced lac repressor and/or tet activator...
7.5K
A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer11:53

A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer

18.9K
This protocol describes the steps for cloning multiple single guide RNAs into one guide RNA concatemer vector, which is of particular use in creating multi-gene knockouts using CRISPR/Cas9 technology. The generation of double knockouts in intestinal organoids is shown as a possible application of this...
18.9K
Generation of Genetically Modified Mice through the Microinjection of Oocytes10:19

Generation of Genetically Modified Mice through the Microinjection of Oocytes

21.6K
The microinjection of mouse oocytes is commonly used for both classic transgenesis (i.e., the random integration of transgenes) and CRISPR-mediated gene targeting. This protocol reviews the latest developments in microinjection, with a particular emphasis on quality control and genotyping...
21.6K
DNA Vector-based RNA Interference to Study Gene Function in Cancer13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

21.0K
RNA interference (RNAi) possesses many advantages over gene knockout and has been broadly used as a tool in gene functional studies. The invention of DNA vector-based RNAi technology has made long term and inducible gene knockdown possible, and also increased the feasibility of gene silencing in...
21.0K
Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans10:58

Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans

18.2K
We describe a protocol using C. elegans and RNAi feeding libraries that allows automated measurement of multiple parameters such as fluorescence, size and opacity of individual worms in a population. We give one example of a screen to identify genes involved in anti-fungal innate immunity in C.
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関連する実験動画

Updated: Jan 14, 2026

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Published on: November 2, 2020

7.0K

再プログラム可能なDNA自己組み立てを使用する多様で堅牢な分子アルゴリズム

Damien Woods1,2,3, David Doty4,5, Cameron Myhrvold6,7

  • 1California Institute of Technology, Pasadena, CA, USA. damien.woods@mu.ie.

Nature
|March 22, 2019
PubMed
まとめ
この要約は機械生成です。

研究者は複雑なアルゴリズムを実行するための 分子自己組み立てを可能にする 大きなDNAタイルセットを開発しました この画期的な発見は プログラム可能な物質と 進歩した分子機械の道を開きました

さらに関連する動画

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

7.5K
A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
11:53

A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer

Published on: July 12, 2017

18.9K

関連する実験動画

Last Updated: Jan 14, 2026

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Published on: November 2, 2020

7.0K
In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

7.5K
A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
11:53

A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer

Published on: July 12, 2017

18.9K

科学分野:

  • 分子ナノテクノロジー
  • 情報ベースの化学
  • アルゴリズムの自己組み立て

背景:

  • 分子生物学では 化学システムが情報を保存し 処理する能力を示しています
  • アルゴリズム的行動は分子自己組み立てに組み込まれ DNAナノテクノロジーで実験的に示されています
  • 情報技術には複雑性の限界があり,以前はDNAの自己組み立てでは扱われていなかった.

研究 の 目的:

  • プログラム可能なシステムの複雑性の値を超えることができるかどうかを調査する.
  • 多様なアルゴリズムを実行できるDNAタイルのセットを設計し,検証する.

主な方法:

  • DNAタイルセットを設計し,355個の単一鎖のタイルを構成しました.
  • 様々な6ビットアルゴリズムを実装するために再プログラムすることによって,実験的にタイルのセットを検証しました.
  • 高精度でアルゴリズムを実行する 21の異なるDNA回路を構築した.

主要な成果:

  • DNAのタイルセットを 再プログラムして 6ビットアルゴリズムを実装した
  • コピー,ソート,パターン生成,計算を含むアルゴリズムの実行を実証した.
  • 低誤差率で3000分の1未満でした

結論:

  • 分子自己組み立ては プログラム可能な化学システムにおける 信頼性の高い構成要素として機能します
  • 開発されたシステムは,分子計算における複雑性の値を超える可能性を示唆しています.
  • 先進的な分子マシンを開発する 分子プログラマーのための新しい創造的空間を可能にします