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関連する概念動画

Transcription Factors02:16

Transcription Factors

70.8K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
70.8K
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

8.9K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
8.9K
Combinatorial Gene Control02:33

Combinatorial Gene Control

8.6K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
8.6K
Master Transcription Regulators02:23

Master Transcription Regulators

6.1K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.1K
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

2.5K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.5K
General Transcription Factors01:30

General Transcription Factors

5.9K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.9K

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関連する実験動画

Updated: May 5, 2026

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
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Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

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複数の核要因が,免疫グロブリン強化剤の配列と相互作用する.

R Sen, D Baltimore

    Cell
    |August 29, 1986
    PubMed
    まとめ

    研究者らは,免疫グロブリン増強剤に結合する3つのタンパク質を特定しました. 1つのタンパク質であるNF-Aは,すべての細胞のオクターマー配列に結合し,もう1つは重量増強剤とカッパ増強剤の両方の特定のメチル化保護部位に結合する. 3つ目のタンパク質はB細胞にのみ存在し,カッパ増強剤と結合する.

    科学分野:

    • 分子生物学は分子生物学である.
    • 免疫学 免疫学とは
    • 遺伝学 遺伝学とは

    背景:

    • 免疫グロブリン (Ig) 増強剤は,Ig遺伝子発現を制御する重要な規制要素です.
    • これらの増強剤に結合するタンパク質を理解することは,B細胞の発達と機能を解読する鍵です.

    研究 の 目的:

    • 免疫グロブリン重鎖およびカッパ軽鎖強化剤に特異的に結合するタンパク質を特定し,特徴づけること.
    • これらのDNA結合タンパク質の結合特性と細胞分布を解明する.

    主な方法:

    • 末端にラベルを貼ったDNA断片を用いた電泳性移動シフトアッセイ (EMSA).
    • Ig増強剤および関連するコントロール配列内の特定の配列へのタンパク質結合の分析.
    • 異なる細胞タイプにおけるタンパク質発生の評価.

    主要な成果:

    • 3つの異なるタンパク質が免疫グロブリン強化剤と結合することが判明しました.
    • どこにでも存在する因子であるNF-Aは,Ig変数遺伝子のセグメントと重鎖強化剤のオクターマー配列と結合する.
    • 第二の至るところに存在するタンパク質は,重量増強剤とカッパ増強剤の両方において,メチル化で保護された部位に対して高い特異性を示す.
    • 第三のタンパク質はB細胞に限定され,カッパ増強剤の配列とSV40増強剤の同一の配列に結合する.

    さらに関連する動画

    Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
    13:55

    Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

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    Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
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    Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

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    関連する実験動画

    Last Updated: May 5, 2026

    Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
    11:02

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    Published on: April 29, 2011

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    Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
    13:55

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    Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
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    結論:

    • 多数の配列特異性DNA結合タンパク質は,免疫グロブリン増強剤の活性を調節する.
    • B細胞特異因子を含む特定されたタンパク質は,Ig遺伝子の転写制御において重要な役割を果たしています.
    • 独特の結合特性により,B細胞特有の遺伝子発現を調節する様々なメカニズムが示唆されています.