非対称なカスケード反応のための設計されたオキシダース-ペロキシゲナスの相互利益システムを利用する
Da Yu1,2, Jian-Bo Wang1,2, Manfred T Reetz3,4
1Key Laboratory of Phytochemistry R&D of Hunan Province, College of Chemistry and Chemical Engineering , Hunan Normal University , 410081 Changsha , People's Republic of China.
Journal of the American Chemical Society
|March 29, 2019
まとめ
P450酵素を用いた新しい生物触媒システムは,3-フェニルプロピオン酸から (R) -フェニルグリコールを生成する. このシステムは,過酸化水素をリサイクルし,ワンポット合成の安全性と効率性を高めます.
科学分野:
- 生物触媒
- 酵素工学
- 有機合成
背景:
- 持続可能な化学合成には 効率的な生物触媒カスケードの開発が不可欠です
- P450酵素は多用途の触媒作用がありますが,H2O2のような反応性中間物質の慎重な管理が必要です.
研究 の 目的:
- 生物触媒カスケードのための相互性のあるP450モノオキシゲネーゼ-ペロオキシゲネーゼシステムを設計する.
- カーボキシル酸から (R) -フェニルグリコールおよびその誘導体のエナンチオセレクティブ合成を達成する.
主な方法:
- P450モノオキシゲナーゼ (P450-BM3) とP450ペロキシゲナーゼ (OleTJE) を含む生物触媒カスケードの構築.
- スタイレン中間物のエナンチオセレクティブエポキシデーションのためのP450-BM3の誘導進化.
- エポキシードヒドローラゼ (ANEH) を利用して水解環を開く.
主要な成果:
- H2O2の再利用を可能にする新しいP450相互利益システムが確立されました.
- (R) - フェニルグリコールと9つの誘導体の合成において,高いエナチオ選択性が達成された.
- カーボキシル酸からキラルアルコールへの1ポット合成が実証された.
結論:
- 開発されたP450システムは,キラルグリコールを生成するための安全で効率的な方法を提供します.
- このアプローチはH2O2の蓄積を最小限に抑え,潜在的な毒性を防止します.
- この戦略は,様々なキラルアルコール誘導体の合成に適用できます.
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