細胞に浸透するポリ硫化物の成長と分解の単分子運動
Gökçe Su Pulcu1, Nicole Stéphanie Galenkamp2, Yujia Qing1
1Department of Chemistry , University of Oxford , Oxford OX1 3TA , United Kingdom.
Journal of the American Chemical Society
|April 2, 2019
まとめ
細胞に浸透する生物分解性ポリ硫化物 (CPD) が治療効果を発揮します. 合成と分解運動によって制御されるそれらの長さは,セルラーターゲティングを決定し,自己サイクルが主要な分解経路である.
科学分野:
- ポリマー化学
- バイオマテリアル科学
- ナノテクノロジー
背景:
- 標的細胞への治療薬の投与は複雑である.
- 生物分解性細胞浸透ポリ硫化物 (CPD) は,細胞浸透および細胞内標的化のための解決策を提供します.
- ポリマーの長さは,細胞の局所化と標的配送の有効性に重大な影響を及ぼします.
研究 の 目的:
- CPDポリマーの成長と分解の動態を調査する.
- 特定の合成条件下で達成可能な鎖の長さを予測する.
- CPD の主要な細胞内デポリメリゼーション経路を解明する.
主な方法:
- 単一分子ナノ反応器を使って CPDの運動を監視した
- ポリマーの成長と分解の速度を測定した.
- 合成とデポリメリゼーションの速度定数
主要な成果:
- 200 mMのディチオラン濃度で,40ユニットのCPDが迅速に合成される (pH7. 4で1秒,pH8. 4で0. 3秒).
- 速度定数は,異なる条件下で達成可能な鎖の長さを予測します.
- 細胞内分解は主にチオールによる鎖内分裂ではなく,自己循環によって起こります.
結論:
- CPDの長さは,合成条件によって正確に制御できます.
- 分解運動を理解することは 標的の薬物投与を最適化するために不可欠です
- 自己循環は,細胞内のCPD脱ポリマー化の支配的なメカニズムである.
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