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関連する概念動画

Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

8.7K
Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
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Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

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Ligand Binding and Linkage00:49

Ligand Binding and Linkage

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Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
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Energy-releasing Steps of Glycolysis01:28

Energy-releasing Steps of Glycolysis

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Glycolysis is divided into two phases based on whether energy is utilized or released. While the first phase consumes ATP, the second phase produces energy in the form of ATP and NADH. The energy is released over a sequence of reactions that turns G3P into pyruvate. The energy-releasing phase—steps 6-10 of glycolysis—occurs twice, once for each of the two 3-carbon sugars produced during steps 1-5 of the first phase.
The first energy-releasing step—the 6th step of glycolysis...
146.5K
Metal-Ligand Bonds02:51

Metal-Ligand Bonds

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The hemoglobin in the blood, the chlorophyll in green plants, vitamin B-12, and the catalyst used in the manufacture of polyethylene all contain coordination compounds. Ions of the metals, especially the transition metals, are likely to form complexes.
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...
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The ADP/ATP Carrier Protein01:42

The ADP/ATP Carrier Protein

4.2K
ADP/ATP carrier or AAC protein is the most abundant carrier protein in the inner mitochondrial membrane. It transports large quantities of ADP and ATP, equivalent to the average human body weight, every day. Among other transporters, ACC protein is one of the best-studied members of the mitochondrial carrier protein family. The ADP/ATP carrier protein comprises two transmembrane helices connected to a loop and a single alpha-helix on the matrix side. It switches between two conformational...
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関連する実験動画

Updated: Jan 26, 2026

Assay for Pathogen-Associated Molecular Pattern PAMP-Triggered Immunity PTI in Plants
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Assay for Pathogen-Associated Molecular Pattern PAMP-Triggered Immunity PTI in Plants

Published on: September 9, 2009

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リガンド誘発アロステリックADP放出プライム 植物NLR複合体

Jizong Wang1,2, Jia Wang2, Meijuan Hu1

  • 1State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Academy of Seed Design, Chinese Academy of Sciences, 100101 Beijing, China.

Science (New York, N.Y.)
|April 6, 2019
PubMed
まとめ

ZAR1のような植物 NLR 免疫受容体は,病原体エフェクタによって活性化されます. 構造研究は,PBL2UMPがRKS1に結合してZAR1を安定させ,ADPの結合を阻害し,植物の防御を開始する方法を明らかにしています.

さらに関連する動画

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
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Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

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Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
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Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

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関連する実験動画

Last Updated: Jan 26, 2026

Assay for Pathogen-Associated Molecular Pattern PAMP-Triggered Immunity PTI in Plants
08:45

Assay for Pathogen-Associated Molecular Pattern PAMP-Triggered Immunity PTI in Plants

Published on: September 9, 2009

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Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
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Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

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Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
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Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

Published on: February 18, 2014

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科学分野:

  • 植物免疫
  • 植物 防御 の 分子 機構
  • 構造生物学

背景:

  • ヌクレオチド結合 (NB),レウシン豊富なリピートレセプター (LRR) (NLRs) は植物免疫反応に不可欠である.
  • Xanthomonas campestrisエフェクタ AvrACは,アラビドプシスのPBL2キナーゼをPBL2UMPに改変し,ZAR1 NLR受容体を活性化する.

研究 の 目的:

  • ZAR1-RKS1とZAR1-RKS1-PBL2UMPの冷凍電子顕微鏡構造を決定する
  • PBL2UMPによるZAR1活性化の構造的基礎を解明する.

主な方法:

  • 電子冷凍顕微鏡 (EM冷凍)
  • タンパク質複合体の構造分析

主要な成果:

  • ZAR1-RKS1 (不活性) とZAR1-RKS1-PBL2UMP (中間状態) の構造を決定した.
  • ZAR1 LRRドメインは,植物においてユニークな形状を採用し,ZAR1を非活性状態で隔離する.
  • RKS1はPBL2UMPの認識を介し,RKS1の活性化セグメントを安定させ,ZAR1のADP結合を阻害する.
  • PBL2UMP結合は,ZAR1 NB領域の柔軟性を誘導する.

結論:

  • ZAR1-RKS1-PBL2UMP構造は,プラントのNLR活性化を理解するためのテンプレートを提供します.
  • ZAR1の機能に関する構造的な洞察は 植物の免疫信号伝達経路に関する知識を深めています