免疫を与える植物NLRレジストソームの復元と構造
Jizong Wang1, Meijuan Hu2, Jia Wang1
1Beijing Advanced Innovation Center for Structural Biology, Tsinghua-Peking Joint Center for Life Sciences, Center for Plant Biology, School of Life Sciences, Tsinghua University, 100084 Beijing, China.
Science (New York, N.Y.)
|April 6, 2019
まとめ
植物免疫受容体,核酸結合,ルシンの豊富なリピート受容体 (NLRs) は,活性化時にペンタメリックZAR1レジストソームを形成する. この構造は,プラズマ膜と結合することで, 植物免疫と病気に対する抵抗を誘発します.
科学分野:
- 植物分子生物学
- 植物免疫学
- 構造生物学
背景:
- ヌクレオチド結合性,ルシンの豊富なリピートレセプター (NLR) は,病原体エフェクターを感知する植物免疫に不可欠です.
- 植物のNLR活性化とレジストソーム形成の生化学的メカニズムは十分に理解されていません.
研究 の 目的:
- 植物のNLR活性化の生化学的メカニズムと構造的基礎を解明する.
- ZAR1レジストソーム複合体と植物免疫反応におけるその役割について説明する.
主な方法:
- 活性ZAR1複合体をRKS1,PBL2,dATPで再構成する.
- クリオ電子顕微鏡でZAR1レジストソームの構造を決定する.
主要な成果:
- 免疫活性化中にZAR1複合体のオリゴメリゼーションが実証された.
- 輪のようなペンタミックZAR1レジストソーム構造を明らかにした.
- 機能的なN端 αヘリックスを形成する構造的改造と折り切りを特定した.
結論:
- ZAR1レジストソームペンタメリゼーションは,核酸結合領域とコイルドコイル領域の両方を含む.
- 活性化中の構造の変化は,プラズマ膜結合,細胞死,および疾患耐性にとって不可欠なN端の螺旋を放出します.
- 免疫における植物レジスタソームの生化学的機能に関する洞察を 提供している.
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