関連する実験動画
Updated: Jan 26, 2026
09:00
Evaluating Plasmonic Transport in Current-carrying Silver Nanowires
Published on: December 11, 2013
5.6K
微生物 の ナノ ワイヤ の 構造 は,電子 を マイクロメーター 上 に 運ぶ 積み重なっ た ヘム を 明らかに し て い ます
Fengbin Wang1, Yangqi Gu2, J Patrick O'Brien3
1Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA.
Cell
|April 6, 2019
まとめ
電子輸送に不可欠な微生物ナノワイヤは 驚くべきことにPilaではなくOmcSタンパク質から 組み立てられています このOmcS構造は,高伝導性と細菌の呼吸における役割を説明する.
科学分野:
- 微生物学
- バイオ物理学
- 構造生物学
背景:
- Geobacter sulfurreducensは,長距離電子輸送 (> 10μm) のために微生物ナノワイヤと呼ばれるタンパク質フィラメントを使用しています.
- これらのナノワイヤは以前はPilaタンパク質からなるタイプIVのピリと特定されていた.
研究 の 目的:
- 微生物ナノワイヤの組立と機能の構造的基礎を決定する.
- これらの電子伝送ネットワークの高伝導性に起因するタンパク質の組成を解明する.
主な方法:
- クリオ電子顕微鏡を用いて,高解像度構造 (3.7 Å) のゲオバクター・スルファルリュケンスナノワイヤを決定した.
- 野生型と変異型株 (ΔomcS) で比較伝導性を測定した.
主要な成果:
- この研究では,Geobacter sulfurreducensのナノワイヤは主にPilaではなく,ヘキサヘムサイトクロームOmcSで構成されていることが明らかになりました.
- 構造はOmcSのマイクロメートルの長さのポリメリゼーションを示し,ヘムが密接に詰め込まれている (∼3.5-6 Å) であり,ユニークなサブユニット間のインターフェースである.
- ワイルドタイプのOmcSフィラメントは,ΔomcS株のフィラメントと比較して100倍以上の伝導性を示した.
結論:
- OmcSは,ゲオバクテルの硫黄還元微生物ナノワイヤの重要な構造および伝導成分です.
- OmcSフィラメント内のユニークなヘム包装と調整は,それらの驚くべき電子輸送能力を説明します.
- この発見は 土壌細菌が 細胞外呼吸と 電子の移転を通じて エネルギー共有を行う方法についての洞察を 提供しています
関連する概念動画
01:28Electron Transport Chains
111.9K
The final stage of cellular respiration is oxidative phosphorylation that consists of two steps: the electron transport chain and chemiosmosis. The electron transport chain is a set of proteins found in the inner mitochondrial membrane in eukaryotic cells. Its primary function is to establish a proton gradient that can be used during chemiosmosis to produce ATP and generate electron carriers, such as NAD+ and FAD, that are used in glycolysis and the citric acid cycle.
The ETC is comprised of...
The ETC is comprised of...
111.9K
01:30The Electron Transport Chain
19.8K
The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
19.8K
01:34The Z-Scheme of Electron Transport in Photosynthesis
13.4K
The light reactions of photosynthesis assume a linear flow of electrons from water to NADP+. During this process, light energy drives the splitting of water molecules to produce oxygen. However, oxidation of water molecules is a thermodynamically unfavorable reaction and requires a strong oxidizing agent. This is accomplished by the first product of light reactions: oxidized P680 (or P680+), the most powerful oxidizing agent known in biology. The oxidized P680 that acquires an electron from the...
13.4K
01:46Electron Transport Chain: Complex I and II
18.5K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
18.5K
01:29Electron Transport Chain Components
938
The electron transport chain (ETC) is a crucial metabolic pathway that facilitates energy conversion in prokaryotic and eukaryotic cells. In eukaryotes, the ETC comprises four membrane-associated protein complexes in the inner mitochondrial membrane. In prokaryotes, the ETC in the plasma membrane can vary in composition, with fewer or different complexes depending on the organism and environmental conditions. These complexes transfer electrons from electron donors, such as NADH and FADH2, to...
938
02:28Electronic Structure of Atoms
28.4K
An atom comprises protons and neutrons, which are contained inside the dense, central core called the nucleus, with electrons present around the nucleus. Taking into account the wave–particle duality of electrons and the uncertainty in position around the nucleus, quantum mechanics provides a more accurate model for the atomic structure. It describes atomic orbitals as the regions around the nucleus where electrons of discrete energy exist, characterized by four quantum...
28.4K