exRNA アトラスの分析により,ヒトの生物流体全体に存在する異なった細胞外RNA 貨物タイプとその媒体が明らかになった
Oscar D Murillo1, William Thistlethwaite1, Joel Rozowsky2
1Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
Cell
|April 6, 2019
まとめ
exRNA アトラスは,細胞外RNA (exRNA) プロファイルを使用して細胞の通信をマッピングします. 新しい解凍モデルにより,生物流体全体に 6 つの exRNA 貨物タイプが特定され,exRNA 機能の理解が進んでいます.
科学分野:
- 生物化学
- ゲノミクス
- 細胞生物学
背景:
- 細胞同士のコミュニケーションは 生物学的プロセスに不可欠です
- 細胞外RNA (exRNA) は細胞間信号伝達において重要な役割を果たします.
- exRNA通信を研究するための既存のリソースは限られている.
研究 の 目的:
- exRNAによって媒介される細胞間通信の包括的な地図を開発する.
- exRNAプロフィールを分析するためのツールを作成し,提供します.
- exRNAの貨物タイプと輸送機について標準化されたモデルを確立する.
主な方法:
- 5309のexRNA-seqおよびqPCRプロファイルを含むexRNAアトラスリソース,バージョン4P1を使用しました.
- exRNAプロファイル間のバリエーションを分析するために,計算式デコンボリューションを適用した.
- 膀性および非膀性 exRNA キャリアの統合分析
主要な成果:
- 6つの異なるexRNA貨物タイプ (CT1-CT4) を識別するモデルを開発した.
- これらの貨物タイプは,血清,プラズマ,CSF,唾液,尿を含む複数の生物流体で検出されました.
- 5つの貨物タイプは,既知のexRNAキャリア (ベジキュラー,リポプロテイン,リボヌクレプロテイン) と関連付けられました.
結論:
- 開発されたデコンボリューションモデルとexRNAアトラスは,exRNA媒介の細胞通信を研究するための堅固な枠組みを提供します.
- このモデルは,生理学的な研究で以前未発見の経路を成功裏に特定しました.
- ケース・コントロール研究において広範に適用できる解消と分析のツールが提供されています.
関連する概念動画
RNA-seq
10.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.8K
Overview of Exosomes
3.1K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
3.1K
Ribosome Profiling
3.8K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.8K
Nuclear Export of mRNA
8.1K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
8.1K
Regulated mRNA Transport
6.6K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
6.6K


