単細胞解像度でのマウスの腸内皮の新興の風景

Sonja Nowotschin¹, Manu Setty², Ying-Yi Kuo¹

¹Developmental Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, USA.

Nature

|April 9, 2019

まとめ

この研究では,単細胞のトランスクリプトームを用いて哺乳類の内皮の発達をマッピングし,エピブラスト細胞が2つの重要な時期に内皮を形成し,これらの細胞が早期の腸管のパターンを明らかにしています.

科学分野:

発達生物学
ゲノミクス
細胞生物学

背景:

哺乳類の内皮の早期の発達は,臓器形成に極めて重要です.
細胞の分化と系統の軌道を詳細にマッピングすることが必要である.

研究の目的:

早期発育から妊娠中期までの哺乳類内皮のオントジェニスを描写する.
内皮の発達における時空動態と転写構造を特徴づける.
エピブラストと内皮の系統の関係を解明する

主な方法:

マウスの胚から112,217個の単細胞トランスクリプトームを生成し,分析する.
細胞の分化と発達軌道をモデル化するためのグラフベースのアプローチの適用.
腸内皮質の細胞の運命と腸管のパターンの空間時間的なマッピング

主要な成果:

妊娠中期まですべての内皮集団の転写の風景を定義した.
エピブラストから内皮の分化のための2つの異なる時間点を特定した (ガストルレーション前およびその間).
腸管に収束する内皮細胞に保存されているが系統特有の特徴を明らかにした.
前後軸に沿った地域化された細胞のアイデンティティと,臓器特有の調整パターンを観察した.

結論:

哺乳類の内皮の発達には,明確な分化波と系統の貢献が特徴である.
出現する腸管は,胚性/胚外起源と血統の歴史を反映した空間的なパターンを表しています.
この研究は,初期の内皮の発達と腸管形成の包括的な転写アトラスを提供します.

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