まとめ
核因子はc-fos増強剤に結合するが,その活動はc-fos転写を制御するだけではない. 外皮成長因子および他の薬剤は,異なったメカニズムを通じて転写を活性化し,複雑な遺伝子調節を明らかにします.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝子規制 遺伝子規制
- 細胞シグナル伝達 細胞信号伝達
背景:
- c-fos遺伝子は,刺激に対する細胞の反応において極めて重要です.
- c-fos転写の調節を理解することは,細胞信号伝達経路の解読の鍵です.
研究 の 目的:
- 遺伝子調節におけるc-fos増強剤に結合する核因子の役割を調査する.
- 皮質成長因子 (EGF) と他の薬剤がc-fos転写を調節するメカニズムを解明する.
主な方法:
- 核抽出物は,A431およびHeLa細胞から作られました.
- エレクトロフォレティック・モビリティ・シフト・アッセイ (EMSA) は,エンハンサー・バインディング・アクティビティを検出するために使用されました.
- EGF,TPA,A23187.7の治療後にc-fos遺伝子転写レベルが測定されました.
主要な成果:
- c-fos増強剤に結合する核因子を特定した.
- EGF治療は,c-fos転写とエンハンサー結合活性の両方を増加させましたが,転写が低下した後でも結合活動は高いままでした.
- c-fos転写が欠けているHeLa細胞は,有意な強化剤結合活性を示した.
- TPAとA23187は,強化剤結合活性を増やすことなく,c-fos転写を誘発した.
結論:
- c-fosの転写調節は,強化剤結合因子のレベルにのみ依存するよりも複雑です.
- EGFとTPAのような腫瘍プロモーターは,c-fos転写を活性化するために異なる経路を利用します.
関連する概念動画
Cell Specific Gene Expression
13.3K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.3K
Co-activators and Co-repressors
7.0K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.0K
Combinatorial Gene Control
8.6K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
8.6K
Reporter Genes
11.4K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.4K
MAPK Signaling Cascades
7.3K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
7.3K
Induced Pluripotent Stem Cells
4.8K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
4.8K


