カッパ免疫グロブリン増強剤結合タンパク質Nf-カッパBの誘導性は,翻訳後のメカニズムによって引き起こされる

R Sen, D Baltimore

Cell

|December 26, 1986

まとめ

核因子カッパB (NF-カッパB) は,翻訳後の改変によって活性化されます. このタンパク質は,

科学分野:

分子生物学は分子生物学である.
免疫学免疫学とは
細胞生物学細胞生物学

背景:

核因子カッパB (NF-カッパB) は,カッパ免疫グロブリン強化剤と相互作用する核タンパク質です.
NF-カッパBは,免疫グロブリン軽鎖遺伝子を転写する細胞に存在し,リポポリサッカリド (LPS) によってプレ-B細胞に誘発される可能性があります.

研究の目的:

NF-kappa B.の活性化メカニズムと細胞分布を調査する.
NF-kappa B誘導がBリンパ球細胞に限定されているかどうかを判断する.

主な方法:

細菌のリポポリサッカリド (LPS) とサイクロヘキシミドでプレB細胞を刺激する.
様々な細胞系 (Jurkat T細胞,HeLa非リンパ性細胞) で活性ホルボールエステルを使用してNF-kappa Bを誘導する.

主要な成果:

LPS刺激により,前B細胞における翻訳後の活性化によりNF-kappa Bを誘発する.
NF-kappa Bの超誘導は,LPSとサイクロヘキシミドを併用した治療で起こる.
Phorbolエステルは,T細胞および非リンパ球細胞におけるNF-カッパBを迅速に誘導し,より広範な分布を示しています.

結論:

NF-kappa Bの活性化は,Bリンパ球細胞に限定されていません.
特定の遺伝子発現を制御する転写因子は,広く存在する前駆因子の翻訳後の修正によって活性化することができます.

さらに関連する動画

11:27

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Published on: November 2, 2018

10:57

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

Published on: January 12, 2020

関連する概念動画

02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...

02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...

02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...

01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...

02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...

02:26

NF-kB-dependent Signaling Pathway

関連する実験動画