電圧イメージングのための硫化カルボフローレッセインの合成
Journal of the American Chemical Society
|April 13, 2019
まとめ
変形したカーボフローレスチンを用いて 新しい電圧感受性光染料を開発しました これらの染料は以前の制限を克服し,電圧感度が向上し,典型的な光素の波長を超えたハイブリッド化学遺伝子電圧イメージングのアプリケーションを提供します.
科学分野:
- 有機化学
- 生物物理化学
- 分子イメージング
背景:
- カーボフローレッセインは赤色シフトスペクトルを有しているが,高pKaとサイクリングの問題を抱えている.
- 既存の電圧感受性プローブには,スペクトル範囲または安定性に関する制限があります.
- 光素の誘導体は広く使用されているが,刺激/放出プロファイルはより短い波長に限られている.
研究 の 目的:
- 変形したカーボフローレセイン・スキャフォールドを基に,新しい電圧感受性光インジケーターを設計し,合成する.
- カーボフローレッセインの限界を克服するために,特に高いpKaとサイクリング.
- 典型的な光器の波長を超えた電圧画像に適した探査機を開発する.
主な方法:
- 塩素化と硫化によるカルボフッ素素の改変
- ハロゲン化硫化カルボフローレセインの合成経路の開発
- 変形された染料をフェニレン・ビニレン分子ワイヤの支架に組み込み,カーボ電圧感受性フッ素光体 (カーボVF染料) を生成する.
- pKa,スペクトルプロファイル,および電圧感受性を含む染料の特性.
- エステラゼ活性化プロダイを使用したフッ素性ラベリング戦略の実証.
主要な成果:
- 塩素化により,カルボフッ素セインのpKa値が5. 2に低下し,硫化によりサイクル化が防止された.
- 新しいカーボVF染料,特にカーボVF2.1 ((OMe).Clは,560 nm以上の刺激/放出を示す.
- 高電圧感度 (100 mVあたり30% ΔF/F) を達成した.
- 緑色の光タンパク質のような他の光物質と互換性があります.
- プロ染色戦略により,エステラゼ活性化による光回復を in vitro および 生きている細胞/ニューロンで可能にしました.
結論:
- 塩素硫化カルボフローレッセインは,電圧感受性染料の有望な新種である.
- これらの染料はより長い波長で電圧イメージングを可能にし,既存の探査機の限界を克服します.
- 開発された探査機は,ハイブリッド化学-遺伝的電圧イメージングアプリケーションに適しています.
関連する概念動画
Voltage
4.1K
The movement of electrons in a conductor requires some form of energy or work, usually provided by an external force, like a battery. This force is called the electromotive force or voltage. The voltage between two points, referred to as points "a" and "b," in an electric circuit is the energy (or work) needed to move a unit charge from point "a" to point "b," and this relationship is expressed mathematically as
4.1K
Electrophilic Aromatic Substitution: Sulfonation of Benzene
7.9K
Sulfonation of benzene is a reaction wherein benzene is treated with fuming sulfuric acid at room temperature to produce benzenesulfonic acid. Fuming sulfuric acid is a mixture of sulfur trioxide and concentrated sulfuric acid.
7.9K
Voltage Dividers
1.3K
In electrical circuits, resistors can be connected in series, sequentially linked one after the other. In a series configuration, the same current flows through each resistor. Ohm's law is a fundamental principle to understand the behavior of resistors in series. It expresses the voltage across these resistors in terms of the current and resistance.
Kirchhoff's voltage law implies that the sum of the voltages across the resistors in series equals the source voltage. This means that the current...
Kirchhoff's voltage law implies that the sum of the voltages across the resistors in series equals the source voltage. This means that the current...
1.3K
Three-Phase Voltages
560
A three-phase generator produces three voltages that are equal in magnitude but have a phase difference of 120 degrees. This identical magnitude and equal phase separated voltages are known as the balanced voltages and help to minimize power loss while ensuring a steady delivery of energy to connected loads. As voltage sources in a three-phase system can be configured in a wye or a delta formation, the loads connected to these systems can also be arranged in either configuration. This...
560
Multiple Voltage Sources
1.7K
Generally, a single battery is not enough to power some devices. In such cases, batteries can be combined in two ways: in series or in parallel.
In series, the positive terminal of one battery is connected to the negative terminal of another battery. Hence, the voltage of each battery is added to give the net voltage, which is increased because each battery boosts the electrons that enter it. The same current flows through each battery because they are connected in series.
Batteries are...
In series, the positive terminal of one battery is connected to the negative terminal of another battery. Hence, the voltage of each battery is added to give the net voltage, which is increased because each battery boosts the electrons that enter it. The same current flows through each battery because they are connected in series.
Batteries are...
1.7K
Dehydration Synthesis
149.2K
Overview
Dehydration synthesis (also called a condensation reaction) is the chemical process in which two molecules covalently link together to form a new molecule, along with the release of a water molecule. Many physiologically important compounds form by dehydration synthesis reactions, such as complex carbohydrates, proteins, DNA, and RNA.
Synthesis of carbohydrates
Sugar molecules are covalently linked together by dehydration synthesis. During the reaction, the hydroxyl (-OH) group from...
Dehydration synthesis (also called a condensation reaction) is the chemical process in which two molecules covalently link together to form a new molecule, along with the release of a water molecule. Many physiologically important compounds form by dehydration synthesis reactions, such as complex carbohydrates, proteins, DNA, and RNA.
Synthesis of carbohydrates
Sugar molecules are covalently linked together by dehydration synthesis. During the reaction, the hydroxyl (-OH) group from...
149.2K


