ハイブリッド マルチプレキス 彫刻光顕微鏡を用いたマウスの脳内イメージング
Siegfried Weisenburger1, Frank Tejera1, Jeffrey Demas1
1Laboratory of Neurotechnology and Biophysics, The Rockefeller University, New York, NY, USA.
Cell
|April 16, 2019
まとめ
この研究は,神経科学のための新しい光学顕微鏡設計を導入し,カルシウム画像の速度と深さを向上させます. この新しいシステムは 覚醒したマウスの神経活動を 高精度で記録します
科学分野:
- 神経科学
- 光学顕微鏡
- 生物医学工学
背景:
- 2フォトンスキャニング顕微鏡は 神経科学に不可欠ですが 視野,速度,深さの制限があります
- 分散した脳組織は 画像処理能力を制限し ニューラル活動の分析を妨げます
研究 の 目的:
- 新しい光学顕微鏡のパラダイムを開発し,生物学的情報収集とニューロン信号の信頼性を最適化します.
- 細胞内神経科学の研究における現在のカルシウム画像技術における限界を克服する.
主な方法:
- 統合されたシステム全体の最適化と技術革新
- ハイブリッドマルチフォトンの記録
- 柔軟な構成のためのモジュール設計.
主要な成果:
- 1 x 1 x 1. 22mmの単細胞解像度で神経活性の体積記録を達成しました.
- 覚醒しているマウスで最大17Hzの獲得速度を有効にします.
- 脳内の複数の領域で 12,000個のニューロンを 画像化しました
結論:
- 新しいイメージングシステムは vivoカルシウムイメージングの能力を大幅に向上させます.
- 脳の深部画像と 大規模なニューラル集団の記録の可能性を示した.
- 脳の様々な領域の 神経回路を研究する強力なツールです
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