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Updated: Jan 26, 2026

Discovering CsgD Regulatory Targets in Salmonella Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing ChIP-seq
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Discovering CsgD Regulatory Targets in Salmonella Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing ChIP-seq

Published on: January 18, 2020

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DISCOVER-Seqを用いてCRISPRのオフターゲットをインビオで無偏見で検出する

Beeke Wienert1,2,3, Stacia K Wyman1, Christopher D Richardson1,2

  • 1Innovative Genomics Institute, University of California Berkeley, Berkeley, CA 94704, USA.

Science (New York, N.Y.)
|April 20, 2019
PubMed
まとめ

私たちはDISCOVER-Seqを開発しました CRISPR-Casゲノム編集による 標的外効果を直接 vivoで検出する 新しい方法です このアプローチはDNA修復因子MRE11を追跡し,細胞と生物におけるCas活性を正確に単基分解します.

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