細胞間命令組成はタンパク質の動態を模倣し,細胞の球体を作り出す
Huaimin Wang1, Zhaoqianqi Feng1, Bing Xu1
1Department of Chemistry , Brandeis University , 415 South Street , Waltham , Massachusetts 02454 , United States.
Journal of the American Chemical Society
|April 30, 2019
まとめ
研究者たちは 酵素で指示された自己組織化ペプチドを開発し ファイブロネクチンの展開を模倣しました このペプチドアセンブリは 細胞シートを球状に変換し 細胞の行動に新たなコントロールを与えます
科学分野:
- バイオマテリアル科学
- 細胞生物学
- 生物化学
背景:
- 細胞媒介による細胞外マトリックス (ECM) の改造は細胞機能にとって極めて重要です.
- 合成材料でECMタンパク質のダイナミックな性質を模倣することは依然として大きな課題です.
研究 の 目的:
- 合成ペプチドアセンブリを開発し,ECMのタンパク質ダイナミクスを模倣する.
- このアセンブリが 細胞の形状と行動を 制御する方法を説明します 特に細胞の球形形成です
主な方法:
- 酵素で指示された フォスフォペプチドの自己組み立てが ナノ粒子 そして ナノファイバーに
- 細胞間空間におけるペプチド脱酸化を触媒化するためにフォスファターゼを使用する.
- ECMコンポーネントとの構造活動関係と相互作用を調査する.
主要な成果:
- ペプチドアセンブリの細胞間形態学的移行は,フィブロネクチンの展開を成功裏に模倣した.
- HS-5細胞の単層から細胞球状の形成は,酵素指示による自己組み立てによって達成された.
- タンパク質分解の安定性,脱リン酸化,およびバイオチン結合は,球形形成の重要なペプチド特性として識別された.
結論:
- この研究は,同型前体からの細胞間指示組成の最初の例を示しています.
- この発見は,タンパク質のダイナミクスを模倣し,細胞の行動を制御するために,細胞反応性ペプチドアセンブリを用いた新しいアプローチを示しています.
- 培養されたペプチド組は,細胞の球形形成を促すため,ラミリンやコラーゲンなどのECM成分と相互作用する.
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