DNA N6-アデニンメチルトランスフェラーゼ複合体の識別とそのクロマチン組織への影響
Leslie Y Beh1, Galia T Debelouchina2, Derek M Clay3
1Departments of Biochemistry & Molecular Biophysics and Biological Sciences, Columbia University, New York, NY 10032, USA; Department of Chemistry, Princeton University, Princeton, NJ 08544, USA; Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ 08544, USA.
Cell
|May 21, 2019
まとめ
研究者は,DNA N6-アデニンメチル化 (6mA) 複合体,MTA1cをシリエットで特定しました. この複合体は性サイクルに不可欠であり,核細胞の占有に影響を与えることでクロマチンの組織に影響を与えます.
科学分野:
- エピジェネティクス
- 分子生物学
- ユカリオットの遺伝子調節
背景:
- DNA N6-アデニンメチル化 (6mA) は,真核生物全体で見られる新生エピジェネティックマークである.
- 染色体組織と生物学的過程におけるその役割は,多くの系統で不完全である.
研究 の 目的:
- 毛皮動物におけるDNA6mAメチルトランスフェラーゼ複合体を特定し,特徴づけること.
- 毛皮性生殖とクロマチンの構造における6mAの機能的意義を調査する.
主な方法:
- 毛皮動物におけるMTA1cメチルトランスフェラーゼ複合体の識別と特徴付け
- オキシトリチャのMTA1触媒サブユニットの遺伝的破壊
- 6mAの改変によるOxytricha染色体のインビトロ再構成
- 核細胞の占有率とクロマチンの改造剤の効果の評価
主要な成果:
- MT-A70とホメオボックスタンパク質で構成される新しいDNA 6mAメチルトランスフェラーゼ複合体,MTA1cがシリアスで特定されました.
- オキシトリチャにおけるMTA1の破壊は,全ゲノムにわたる6mAを廃止し,性サイクル完了を阻害した.
- 6mAは配列独立の方法で直接核細胞結合を阻害することを実証した.
- クロマチンのリモデレータACFは6mAのヌクレオソーム不利な効果に対抗することができます.
結論:
- この研究では,シリエットのDNA N6-アデニンメチルトランスフェラーゼの分岐を特定した.
- DNA6mAは,核細胞の占有率を調節することによって,毛皮性繁殖とクロマチンの組織に重要な役割を果たします.
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