Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Initiation of Translation02:33

Initiation of Translation

38.5K
Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
38.5K
Initiation of Translation02:33

Initiation of Translation

8.0K
8.0K
Handwashing II: Pre-procedure and Initial Procedure Steps01:19

Handwashing II: Pre-procedure and Initial Procedure Steps

1.5K
The pre-procedure steps of handwashing include removing jewelry and rolling up sleeves. However, many organizations allow staff to wear wedding rings.
The hand washing procedure itself includes the following steps. First, cover cuts, if any, on hands with a waterproof dressing. Cuts and abrasions can become contaminated with bacteria hindering the ability to clean the area thoroughly. In addition, repeated hand washing can worsen an injury.  The nails must be short and clean, without nail...
1.5K
Translation01:31

Translation

156.0K
Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of...
156.0K
Translation01:31

Translation

17.7K
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Proteins are...
17.7K
Termination of Translation01:44

Termination of Translation

27.5K
The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...
27.5K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Environment and reproductive health in China: challenges and opportunities.

Environmental health perspectives·2012
Same author

Posttransplant mortality risk assessment for adult-to-adult right-lobe living donor liver recipients with benign end-stage liver disease.

Scandinavian journal of gastroenterology·2012
Same author

Sodium nitrite protects against kidney injury induced by brain death and improves post-transplant function.

Kidney international·2012
Same author

OIC-A006-loaded true bone ceramic heals rabbit critical-sized segmental radial defect.

Die Pharmazie·2012
Same author

Liquid chromatography-mass spectrometric multiple reaction monitoring-based strategies for expanding targeted profiling towards quantitative metabolomics.

Current drug metabolism·2012
Same author

Structural and functional characterization of mature forms of metalloprotease E495 from Arctic sea-ice bacterium Pseudoalteromonas sp. SM495.

PloS one·2012

関連する実験動画

Updated: Jan 24, 2026

Preparation of High-Temperature Sample Grids for Cryo-EM
05:05

Preparation of High-Temperature Sample Grids for Cryo-EM

Published on: July 26, 2021

4.2K

バクテリアの翻訳開始の遅い段階は,時間解析の冷凍-EMを使用して可視化されています.

Sandip Kaledhonkar1, Ziao Fu2, Kelvin Caban3

  • 1Department of Biochemistry & Molecular Biophysics, Columbia University, New York, NY, USA.

Nature
|May 21, 2019
PubMed
まとめ

この研究は,時間分解の冷凍EMを用いた細菌70S開始複合体の形成中の動的形状の変化を明らかにしています. 初期因子とfMet-tRNAfMetがトランスレーション延長のためにどのように再編成されるかを視覚化しています.

さらに関連する動画

Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy
10:05

Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy

Published on: November 6, 2021

4.7K
Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton
08:59

Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton

Published on: February 25, 2021

4.2K

関連する実験動画

Last Updated: Jan 24, 2026

Preparation of High-Temperature Sample Grids for Cryo-EM
05:05

Preparation of High-Temperature Sample Grids for Cryo-EM

Published on: July 26, 2021

4.2K
Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy
10:05

Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy

Published on: November 6, 2021

4.7K
Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton
08:59

Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton

Published on: February 25, 2021

4.2K

科学分野:

  • 分子生物学
  • 構造生物学
  • 生物化学

背景:

  • バクテリアの翻訳開始には,30Sおよび50Sサブユニット,イニシアターtRNAおよび因子から70S複合体を形成することが含まれます.
  • 70S複合体が伸びるには,初期因子が解離しなければならないが,ダイナミクスと中間物質は不明である.

研究 の 目的:

  • 70S初期複合体の形成と成熟の過程における構造変化と中間物質の解明
  • バクテリアの翻訳開始の制御におけるダイナミクスの役割を理解する.

主な方法:

  • 時間解像度の高い冷凍電子顕微鏡 (TR-cryo-EM) を使用して,原子に近い解像度のスナップショットを撮影した.
  • 分析はリボソームの構成的再編成,イニシアーション因子,fMet-tRNAfMetに焦点を当てた.

主要な成果:

  • 形状の変化の連続を視覚化して 亜単位結合と因子解離を誘導した
  • 成熟時にfMet-tRNAfMetの動的再配置を延長適合状態に捉えた.

結論:

  • TR-cryo-EMは 70S複合体の形成のメカニズムと規制に関する前例のない洞察を提供します.
  • この研究は,細菌の翻訳開始と延長能力のダイナミックなプロセスを明確にします.