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Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules
Published on: July 25, 2013
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市民科学者によるデノボのタンパク質設計
Brian Koepnick1,2, Jeff Flatten3, Tamir Husain3
1Department of Biochemistry, University of Washington, Seattle, WA, USA.
Nature
|June 7, 2019
まとめ
市民科学者はFolditゲームを用いて 新しいタンパク質を設計し 自然界では見られない新しい折りたたみを含む 多様な構造を作り出しました これは新しいタンパク質のデザインに 市民科学の創造的な問題解決の力を 示しています
科学分野:
- 生物化学
- コンピュータ生物学
- 市民科学
背景:
- オンラインの市民科学プロジェクトは通常,データタスクのパターン認識を活用します.
- エテRNAのような以前のゲームは RNA構造のデザインを調査した.
- 新しいタンパク質の設計は,タンパク質構造の3D性により複雑である.
研究 の 目的:
- Folditゲームを用いて 新しいタンパク質のデザインに 市民科学者の可能性を探求する
- オンラインゲームを通じて 新しいタンパク質の構造と配列を生成する
- プレイヤーが作成したタンパク質の実現可能性と成功率を評価する.
主な方法:
- Folditのオンラインゲームは,プロテインの設計に適応し,拡張ペプチド鎖から折りたたまれたタンパク質構造と配列を作成するようプレイヤーに挑戦しました.
- プレーヤーのデザインが分析され,最高得点のソリューションがゲームの改善を告げた.
- 146のプレーヤーが設計したタンパク質配列が合成され,遺伝子にコードされ,Escherichia coliで発現した.
主要な成果:
- Folditプレーヤーは様々なシリコンタンパク質の構造と 配列を成功裏に生成しました
- 合成された146のデザインのうち56は表現され,溶解し,安定したモノメリック折りたたみ構造を採用した.
- デザインは20の異なるタンパク質の折りたたみを示し その中には自然界で観測されていなかった 新しい折りたたみも含まれていました
- 4つのデザインの高解像度構造は プレイヤーが作成したモデルと密接に一致しました
結論:
- 市民科学者は 新しいタンパク質のデザインに 貢献できるのです
- Folditプラットフォームは新しいタンパク質の構造と配列の発見を容易にする.
- このアプローチは 市民科学者の暗黙の知識と 複雑な科学的問題を解決する可能性を 強調しています
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