細胞プラズマ膜の抗原のラベルフリーイメージングのための電気化学発光ベースの電容性顕微鏡

Jingjing Zhang^1,2, Rong Jin¹, Dechen Jiang¹

¹State Key Laboratory of Analytical Chemistry for Life and School of Chemistry and Chemical Engineering , Nanjing University , Nanjing 210023 , P. R. China.

Journal of the American Chemical Society

|June 11, 2019

まとめ

新しい電気化学発光 (ECL) 容量顕微鏡は,種をラベルなしで可視化します. この技術は,容量の変化を感知することで,細胞上の癌胚性抗原 (CEA) を検出し,敏感なバイオ分子検出を可能にします.

科学分野:

分析化学
バイオ物理学
ナノテクノロジー

背景:

容量顕微鏡は通常,視覚化のためのラベルを必要とします.
既存の電気化学発光 (ECL) 方法は,敏感な検出のためにタグを必要とします.
表面や膜に生体分子のラベルのない可視化はまだ課題です.

研究の目的:

電気化学発光 (ECL) ベースの容量顕微鏡を用いた新しいラベルフリー可視化方法を確立する.
電極表面と細胞膜に特定の生物分子の検出を実証する.
バイオアッセイとバイオ分子イメージングのための敏感なプラットフォームを開発する.

主な方法:

ECLベースの容量顕微鏡で正方形の波電圧を使用する.
強化されたECLを誘導するために,種に結合する能力の低下を測定します.
1.5kHzの正方形の波電圧を信号識別のために適用する.
エレクトロッド表面とMCF-7細胞の癌胚性抗原 (CEA) を視覚化する.

主要な成果:

エレクトロッド表面と細胞のプラズマ膜の種をラベルなしで可視化することができました.
開発した顕微鏡を用いて1pgの癌胚性抗原 (CEA) が検出されました.
抗原-抗体複合体の形成後の容量変化による単一のMCF-7細胞におけるCEA抗原の画像化に成功しました.
容量低下による結合位置でのECL信号の強化が実証された.

結論:

レーベルなしの可視化のための前例のないECLベースの容量顕微鏡を確立しました.
この方法は,ECLタグなしで生物分子の敏感な検出と直接のイメージングを可能にします.
このアプローチは,ラベルなしのバイオアッセイと生物分子の敏感なECL検出のための新しい道を提供します.

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