ナノオルグ微生物工場:量子ドットバクテリアナノバイオハイブリッドを用いた光駆動再生可能な生化学合成
Yuchen Ding1,2,3, John R Bertram3,4, Carrie Eckert3,5
1Chemical and Biological Engineering , University of Colorado Boulder , Boulder , Colorado 80303 , United States.
Journal of the American Chemical Society
|June 28, 2019
まとめ
エンジニアリングされたバクテリアは 量子ドット (QD) を利用して 二酸化炭素,水,窒素を バイオ燃料や化学物質に変換します これらのナノバイオハイブリッド生物は 効率的で光で動く ナノ微生物工場として機能します
科学分野:
- バイオテクノロジー
- ナノテクノロジー
- 合成生物学
背景:
- 生きている細胞は ナノスケール素材と 自然に接しないのです
- ナノスケールの構成要素を持つ人工生物は 独特の多機能特性を備えています
- ナノスケールの材料と細胞を インタフェースにするには 化学的,エネルギー的,生物互換性のある設計が必要です
研究 の 目的:
- 細菌と量子ドット (QD) を結合してナノバイオハイブリッド生物 (ナノオーガ) を作る.
- 二酸化炭素,水,窒素を用いた バイオ燃料と化学薬品の生産を可能にします
- QD細胞の結合とナノ臓器の機能のメカニズムを調査する.
主な方法:
- 異なる興奮エネルギーを持つ7つの異なるコアシェル量子ドット (QD) を利用した.
- バクテリアのタンパク質への親和性結合のために,亜鉛に富んだQDシェル面を使用した.
- 細胞の吸収と生存のための組み込まれたシステインズウィットリオンリガンド.
- 照明されたQDは,光誘発の還元反応を誘導する.
主要な成果:
- 細菌の酵素部位とQDを UVから近赤外線スペクトルで成功裏に結合した.
- バイオ燃料 (IPA,BDO,MKs,H2) と化学物質 (FA,NH3,C2H4,PHB) のライト駆動生産が実証されている.
- 約10^6~10^8モルの製品/モルのセルで,高いターンオーバー数 (TON) を達成した.
- ナノ微生物の生存と機能が確認された.
結論:
- QDとバクテリアをインタフェースにすることで 機能的なナノオルガーを開発し,持続可能な化学およびバイオ燃料の生産を実現しました.
- エンジニアリングナノマテリアルを用いた光活性化生物触媒の新しい方法が確立されました.
- 効率的で再生可能エネルギーで動く 細胞工場としてのナノオルガの可能性を強調しました
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