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活性化可能なNIR光/MRIバイモダルプローブ,酵素媒介による光反応と自己組み立てによるin vivoイメージング
Runqi Yan1, Yuxuan Hu1, Fei Liu1
1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering , Nanjing University , Nanjing 210023 , China.
Journal of the American Chemical Society
|June 28, 2019
まとめ
研究者らは,近赤外線の光と磁気共鳴画像を同時に撮るための新しいバイモダルプローブを開発しました. この探知器は,生細胞と腫瘍におけるアルカリリンファスファターゼ (ALP) 活性をリアルタイムで高感度で可視化します.
科学分野:
- 生物医学工学
- 分子イメージング
- ナノテクノロジー
背景:
- 刺激に反応する小さな分子の自己組み立ては,分子イメージングと組織工学にとって有望である.
- 単一の小分子探査機内で同時にマルチモダリティー画像信号を活性化することは大きな課題です.
研究 の 目的:
- 小分子ベースの活性化可能な近赤外線 (NIR) 光と磁気共鳴 (MR) のバイモダルプローブを設計・開発する.
- 強化された分子イメージングのために,酵素反応のインシット自己組み立てと,フッ素反応を統合する.
主な方法:
- 酵素反応自己組み立てを統合したバイモダルプローブ (P-CyFF-Gd) を設計した.
- アルカリリンファスファターゼ (ALP) をモデルターゲットとして利用し,細胞膜に過剰発現した.
- クリオスキャニング電子顕微鏡 (cryo-SEM) を使用した膜局所化された組立ナノ粒子 (NP) を視覚化.
主要な成果:
- ALPの活性化により,同時に70倍以上のNIR光増強と約2. 3倍以上のr1緩解性増強を達成した.
- リアルタイムで高感度で高空間解像度で画像を撮影し,生きた腫瘍細胞とマウスのALPの局所化を可能にしました.
- 肝臓の正体腫瘍を成功裏に特定し,手術中の画像誘導切除を助長した.
結論:
- 開発された戦略は,ALP活動イメージングのインサイト自己組み立てによる活性化可能なNIR光とMRIを効果的に組み合わせています.
- このアプローチは,リアルタイムの in vivo 酵素活性と位置イメージングのための他の活性化可能なビモダルプローブを設計するための汎用性のあるプラットフォームを提供します.
- この探査機は 効率的な画像誘導腫瘍切除を容易にし 臨床応用の可能性を強調しています
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