DNAに結合するFosタンパク質は,酵母における転写を活性化します
1Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
Cell
|January 29, 1988
まとめ
Fosタンパク質は,DNAをターゲットにすると,酵母における遺伝子転写を活性化することができます. この発見は,Fosタンパク質が転写を刺激することによって細胞遺伝子を影響し,さらなる研究を支援することを示唆しています.
科学分野:
- 分子生物学は分子生物学である.
- 腫瘍生成 (オンコゲネシス) について
- イースト遺伝学 イースト遺伝学
背景:
- Fosタンパク質 (v-Fosとc-Fos) は,細胞の調節に関与する腫瘍遺伝子である.
- Fosタンパク質の機能のメカニズムを理解することは,がん研究にとって極めて重要です.
研究 の 目的:
- Fosタンパク質の転写的調節活動を調査する.
- Fosタンパク質がDNAに結合すると遺伝子発現を活性化できるかどうかを判断する.
主な方法:
- バクテリアのLexA抑制体およびv-fos/c-fos腫瘍遺伝子のDNA結合ドメインを使用して,LexA-Fos融合タンパク質を構成した.
- レポーター遺伝子発現を測定することによって,酵母における転写活性化を評価した.
- 異なるDNA結合ドメインと標的遺伝子を用いて特異性をテストした.
主要な成果:
- LexA-Fos融合タンパク質は酵母における転写を活性化させ,ネイティブ酵母活性化剤に匹敵する.
- 活性化は,標的遺伝子の上流にあるLexA結合部位の存在に依存していた.
- v-Mycとc-Mycとの融合タンパク質はより弱い活性化を示したが,LexA単独または他の抑制剤と融合したものは転写を活性化しなかった.
結論:
- Fosタンパク質は,プロモーターDNAに特異的に結合すると,真核生物の遺伝子発現を活性化します.
- このメカニズムは,Fosタンパク質が細胞の遺伝子転写を刺激することによって効果を発揮することを示唆しています.
- 酵母を用いてFosとMycの転写制御を研究することは,より高い生物における遺伝分析の貴重なモデルを提供します.
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